摘要
目的 观察褪黑激素 (MT)对小鼠吗啡戒断反应及血浆、脑组织中NO含量的影响。方法 定量吗啡sc,建立小鼠吗啡依赖模型 ;纳洛酮ip催瘾。用褪黑激素连续ig 3d。根据小鼠戒断反应中出现跳跃反应的潜伏期、跳跃次数、体重丢失评定戒断反应强度。用硝酸还原酶法检测血浆和脑组织中NO含量。结果 用褪黑激素ig可明显延长小鼠吗啡戒断跳跃反应的潜伏期、减少跳跃次数 ,对体重丢失也有所改善。MT可抑制由戒断反应引起的血浆和脑组织中NO含量的升高。结论 褪黑激素可抑制小鼠吗啡戒断反应并降低由戒断反应引起的血浆和脑组织中NO含量的升高。
AIM To observe the effect of melatonin (MT) on morphine withdrawal syndromes and determine the content of NO in plasma and brain tissue in morphine dependent mice. METHODS A physical dependent model in mice was established by subcutaneous injection of morphine. MT (15 mg·kg -1 , qd×3) was given by intragastric infusion (ig) for three days. Withdrawal syndromes were induced by intraperitoneal injection of naloxon (5 mg·kg -1 ). The intensity of withdrawal syndromes was evaluated according to the jumping latency, the jumping times and the body weight loss. The content of NO was detected with Griess method. RESULTS The jumping latency of morphine withdrawal reaction was prolonged and the jumping times were reduced obviously by ig MT. The increased NO content in plasma and brain tissue in morphine dependent mice was reduced by ig MT. CONCLUSION The physical withdrawal syndromes and the content of NO in plasma and brain tissue in morphine dependent mice are inhibited by MT.
出处
《药学学报》
CAS
CSCD
北大核心
2002年第3期175-177,共3页
Acta Pharmaceutica Sinica
