摘要
目的 :研究大肠癌患者外周血中 CK2 0 m RNA的阳性表达可作为肿瘤细胞血行播散的标志 ,探讨CK2 0 m RNA阳性表达与肿瘤病理指标的关系 ,观察大肠癌患者手术前、后外周血中 CK2 0 m RNA表达的变化情况。方法 :采用巢式逆转录多聚酶链反应 ( RT- PCR)的方法 ,对大肠癌 4 2例、原发性肝细胞肝癌 5例、对照组 (非癌肿 ) 10例患者手术前、后不同时间外周血中肿瘤细胞的靶基因 CK2 0 m RNA表达进行检测。结果 :对照组 (非癌肿 ) 10例及原发性肝细胞肝癌 5例手术前、后外周血中 CK2 0 m RNA均为阴性表达。大肠癌组 4 2例 ,手术前外周血中 CK2 0 m RNA阳性表达率为 5 0 .0 % ( 2 1/ 4 2 ) ,手术后 4 8h外周血中 CK2 0 m RNA阳性表达率为 5 9.5 % ( 2 5 / 4 2 )。大肠癌患者外周血中 CK2 0 m RNA的阳性表达率与肿瘤细胞病理分化程度无关 ,与 Dukes分期、淋巴结转移、远处转移有关。结论 :大肠癌患者外周血中 CK2 0 m RNA的阳性表达可作为肿瘤细胞血行播散的标志 ,有助于判断大肠癌的预后及鉴别原发性肝细胞肝癌和大肠癌肝转移。手术操作会促使肿瘤细胞向血液中播散 ,手术过程中应加强预防措施以减少癌细胞的播散。
Objective:To expound that the expression of CK20 mRNA in peripheral blood of patients with colorectal carcinoma can be a helpful marker of circulating dissemination of carcinoma cells,to indicate the relations between the expression of CK20 mRNA and pathologic data of these patients,to disclose the effect of surgical manipulation on the expression of CK20 mRNA in peripheral blood of patients with colorectal carcinoma.Methods:Using nested reverse transcriptase-ploymerase chain reaction (RT-PCR) technique,cytokeratin 20(CK20) mRNA in peripheral blood of 42 patients with colorectal carcinoma,5 patients with primary hepatic carcinoma and 10 cases of control group were detected at various time points before and after operation.Results:The expression of CK20 mRNA in peripheral blood of 10cases of control group and 5 patients with primary hepatic carcinoma were all negative.The positive rate of CK20 mRNA in peripheral blood of 42 patients with colorectal carcinoma was 50.0%(21/42) before operation.Two days after operation,the positive rate of CK20 mRNA was 59.5%(25/42).The positive detection of CK20 mRNA in peripheral blood was significantly correlated with Dukes' stage,limp node metastasis and liver metastasis,but had no coincidence with differentiation of colorectal carcinoma.Conclusions:The expression of CK20 mRNA was a helpful marker of circulating dissemination of colorectal cancer cells.It can be helpful to the judgement of progonosis of colorectal carcinoma.Surgical manipulation may provoke cancer cell dissemination,so the preventive measures should be reinforced during operation. Colorectal
出处
《南通医学院学报》
2002年第1期24-26,共3页
ACTA Academiae Medicinae Nantong
