摘要
目的 建立定量测定人血浆中胸腺九肽的毛细管区带电泳法。方法 运行缓冲液为 5 0mmol·L- 1Na2 B4O7 10mmol·L- 1 Na2 HPO4(pH 9 10 )。毛细管总长 5 7cm ,有效长度 5 0cm ,内径 75 μm。运行电压 12kV ,运行温度2 5℃。检测波长 :2 0 0nm。结果 胸腺九肽的线性范围为 1～ 10 μg·mL- 1 ,γ =0 9990 ,重现性RSD为 3 0 5 % ,回收率为70 97% ,RSD为 3 32 %。结论 毛细管电泳法可检测人血浆胸腺九肽的含量 ,方法简便、可靠、快速。
AIM To establish a capillary zone electrophoresis (CZE) method for determination of thymulin in human blood plasma. Thymulin is a nonapeptide produced by the thymic epithelium. METHODS The uncoated capillary used was 75 μm ID×57 cm (50 cm effective length). The experimental conditions were as follows: the running buffer was phosphate-borate buffer containing 50 mmol·L -1 Na 2B 4O 7-10 mmol·L -1 Na 2HPO 4 (pH 9 10); running voltage was 12 kV; operated temperature was 25℃; running time was 13 minutes, detective wavelength was 200 nm. RESULTS The linear range of the thymulin standard curve was 1～10 μg·mL -1 , γ=0 9990, the average recovery was 70 97% with RSD of 3 32%, the RSD of reproducibility was 3 05%. CONCLUSION The method of CZE for determination of thymulin in human plasma is simple and reliable.
出处
《药学学报》
CAS
CSCD
北大核心
2002年第3期214-216,共3页
Acta Pharmaceutica Sinica
