摘要
用 2 0 0 ,4 0 0 ,6 0 0 ,80 0 mg/L 氯化胆碱 (CC)分别处理 (喷施 )两叶期小麦幼苗 ,均可显著降低叶片的相对电导率 ,且以 4 0 0 mg/L CC处理较优 (最多可比对照降低 75 % )。用 4 0 0 mg/L CC处理二叶期小麦幼苗 12 h后再用 2 0 % PEG6 0 0 0胁迫 ,每隔一定时间检测小麦叶片脯氨酸 (Pro)、叶绿素、蛋白质和丙二醛 (MDA)的含量 ,相对含水量 (RWC)及叶片膜相对电导率 ,以及检测 PEG胁迫 2 4 h后小麦叶片质膜 ATPase活性等指标。结果表明 ,经CC处理的小麦幼苗叶片 Pro、叶绿素、蛋白质的含量和 RWC均比对照高 ,而 MDA含量和质膜相对透性比对照低 ,且这些效应随时间延长而增大 ;CC处理过的植株质膜 ATPase活性也高于对照植株。上述结果表明 ,CC可有效缓解渗透胁迫对植物的损伤 。
With 200, 400, 600 or 800 mg/L choline chloride (CC) sprayed in wheat seedlings in two leaf stage, the relative electric conductivity of leaves could be reduced apparently with the largest reduce rate of 75% at the concentration of 400 mg/L. 12 hours after sprayed with 400 mg/L CC, wheat seedlings were stressed by 20% PEG6000, then several indicators including the contents of Proline, chlorophyll, protein and MDA, relative water content (RWC), relative electric conductivity of leaves and the activity of plasma membrane bound ATPase, which was extracted from wheat leaves having been stressed by PEG6000 for 24 hours, were measured at an interval. The obtained results indicated that with the treatment of 400 mg/L CC, the contents of Proline, chlorophyll and protein and RWC of wheat leaves were higher than those of the control, while the MDA content and relative electric conductivity of leaves were lower than those of the control, which were more apparent with the time of treatment, and the ATPase activity of the treated wheat was also higher than that of the control. All the results mentioned above showed that CC could effectively alleviate the damage of plants under osmotic stress and improve the resistance of the plants.
出处
《干旱地区农业研究》
CSCD
北大核心
2002年第1期57-60,共4页
Agricultural Research in the Arid Areas
基金
"8 63"计划资助项目
