摘要
用禽脑脊髓炎病毒 (AEV)不同毒株分别接种易感鸡胚所制作的油乳剂灭活疫苗 ,分别给 4组正常 1周龄雏鸡接种免疫 ,另设 1组SPF雏鸡作空白对照。对免疫前后及攻毒后的雏鸡 ,以琼脂扩散试验 ,淋巴因子的提取 ,血清IgG的分离、纯化及检测 ,鸡胚敏感试验 ,病理学观察为检验手段 ,以期对本油乳剂灭活疫苗的免疫效果作出综合评定 ,同时根据各试验的结果对免疫机理作初步的探讨。结果表明 ,各组试验用疫苗均安全有效 ,雏鸡免疫后体内抗体持续保持高水平 ,雏鸡免疫期至少达 37周 ,子代鸡胚敏感试验保护率≥ 92 .3%以上。免疫机理方面 :以IgG为主的体液免疫在抵抗感染过程中起关键作用 ;细胞免疫也同时存在 ,并与日龄的增长存在一定的相关性 。
Oil emulsion inactivated vaccine was prepared by inoculating susceptible embryoes, with different strains of AEV. Four groups of normal chickens of 2～7 days of age were given injections for immuniation, respectively. Another group is used as control. This study is expected to evaluate the immunological effect and discuss the immunological mechanism by means of five different experiments, i.e.the agar gel precipitin test, the isolation of lymphokine, the isolation, purification and analysis of blood serum IgG, embryo susceptibility test, clinical and pathological examination. The results of these experimernts indicate that oil emulsion inactivated vaccine is safe and effective.The chickens were normal when inoculated with AE strong virus after immunity 4 and 37 weeks. Immunological mechanism is that the humoral immunity plaies an important role and celluar immunity exists, but it is not important in the process of the resistance to AEV.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第3期335-339,共5页
Scientia Agricultura Sinica
