摘要
目的应用纤维蛋白胶 (fibringlue ,FG)、生长激素 (growthhormone ,GH)于腹腔感染条件下的大鼠小肠吻合 ,探讨其有效性和作用机制。方法将大鼠分为正常对照组和在腹腔感染模型下行标准肠吻合组 (丝线吻合 12针 )、简易吻合组 (丝线吻合 4针 )、简易吻合 +FG组、简易吻合 +GH组、及简易吻合 +FG +GH组共 6组 ,于小肠局部切除吻合术后第 1、3、5天分别观察、测定其吻合口不良愈合率、吻合口爆破压、吻合口羟脯氨酸浓度等指标。结果在感染条件下标准吻合组吻合口爆破压于术后第 1天至第 3天分别为 (18± 10 )mmHg和 (36± 11)mmHg ,不良愈合率达 30 % ;而使用FG行胶合组吻合口强度显著提高 ,分别为 (32± 7)mmHg和 (6 5± 9)mmHg ,不良愈合显著减少 ,仅为11 76 %。在胶合的基础上加用GH与未加用GH组比较 ,羟脯氨酸浓度无显著性差异。结论大鼠在腹腔感染条件下可以使用FG实施小肠肠吻合并减少并发症的发生 。
ObjectiveTo study the effect of fibrin glue(FG) and growth hormone(GH) on the anastomosis of the small bowel at peritonitis. MethodsResection and anastomoses of the small bowel at peritonitis was performed in rats. In group N:normal control;in group A:a standard anastomosis was constructed with 12 silk sutures;in group B:an incomplete anastomosis was constructed with only 4 silk sutures;in group C: incomplete anastomosis+FG;in group D: incomplete anastomosis+GH;in group E: incomplete anastomosis+FG+GH.On days 1,3 and 5,ten rats in each group were sacrificed.The healing of anastomosis were evaluated,the anastomotic bursting pressure and hydroxyproline concentration were measured. Results In group A the bursting pressure was low from day 1 to day 3(18±10?mm?Hg and 36±11?mm?Hg each).The percent of false-healing was 30%,significantly higher than group N.The bursting pressure in group C was significantly higher than in group A(32±7?mm?Hg and 65±9?mm?Hg each),the healing of the anastomosis was significantly improved,the percent of false-healing was 11.7%.Hydroxyproline concentration of each group showed no differences. Conclusion Fibrin glue helps the healing of the anastomosis of the small bowel at peritonitis in rats,and can protect it from complications. The addition of GH does not improve healing.
出处
《中华普通外科杂志》
CSCD
北大核心
2002年第3期173-176,共4页
Chinese Journal of General Surgery
基金
全军"十五"医药卫生重点课题 (编号 0 130 12 )
