摘要
探讨氟伐他汀对兔髂动脉内皮剥脱后造成的动脉粥样硬化模型中细胞增殖的影响。将雄性新西兰兔随机分为氟伐他汀组 (n =2 8)和对照组 (n =2 8) ,用球囊导管剥脱内皮及高胆固醇饮食造成动脉粥样损伤 ,分别在内皮损伤后 1、2、4、8周处死动物并取髂动脉 ,用常规免疫组织化学染色和计算机图像分析方法观察氟伐他汀对新生内膜中平滑肌细胞增殖和血小板源生长因子 BB的影响。结果发现 ,氟伐他汀组内膜中增殖细胞核抗原染色阳性细胞率明显少于对照组 (P <0 .0 5 ) ;氟伐他汀组血小板源生长因子 BB含量一直处于较低水平 ,且明显低于对照组 (P <0 .0 5 ) ;血管内膜增殖细胞核抗原染色阳性细胞率与血小板衍生因子 BB含量的相关系数为 0 .712 7(P <0 .0 1)。提示氟伐他汀可能部分通过抑制血小板源生长因子 BB合成和分泌而抑制平滑肌细胞增殖。
Aim The present study was to investigate the effe cts of Fluva statin on vascular smooth muscle cells (VSMC) proliferation in atherosclerotic model of iliac artery of rabbit after endothelial denudation. Method s The New Zealand White Rabbits were randomized into Fluvastatin group (n=28) and control group(n=28). The atheroscl erotic lesions were developed by inducing endothelial denudation with balloon ca theter and being given high dosag e of cholesterol diet at the same time. The experimental animals were killed an d iliac arteries were removed at 1, 2, 4, 8 week after endothelial injury respect i vely. Through the methods of routine immunohistochemical staining and image ana l ysis system, we observed the effects of Fluvastatin on the expression between pr oliferating cell nuclear antigen (PCNA) and platelet derived growth factor BB (PDGF BB) in neointima. Results Percentages of PCNA postiv e cells in the intima of Fluvastatin group was lower than that in control group apparently. The content of PDGF BB in Fluvastatin group was al ways at a lower level and even lower than that in control group (P<0.05). Coefficient of linear correlation of PCNA with PDGF BB was 0.7127( P<0.01). Conlusion Fluvastatin may partly in hibit VSMC proliferation through decreasing the synthesis of PDGF BB.
出处
《中国动脉硬化杂志》
CAS
CSCD
2002年第1期38-40,共3页
Chinese Journal of Arteriosclerosis
