摘要
目的 :研究氰戊菊酯 (Fen)对雄性生殖内分泌系统的损害作用及其机制。 方法 :将不同剂量的Fen(0、2 .4、12、6 0mg/kg) ,每日分别对雄性成年SD大鼠连续灌胃 ,染毒 15、30d ,应用RIA法测定大鼠血清中FSH、LH、T和睾丸匀浆中T的水平 ,同步测定睾丸标志酶ACP、γ GT的活性 ,并采用精子头计数法观测每日精子生成量 (Spr)的变化。 结果 :与对照组相比 ,染毒 15d时 ,血清中FSH水平在≤ 12mg/kg剂量组均明显升高 (P <0 .0 1) ,血清中LH含量在 12mg/kg剂量组显著增加 (P <0 .0 1) ,而睾丸匀浆中T在≥ 12mg/kg剂量组中表现为显著下降 (P<0 .0 1) ;染毒至 30d时 ,血清中FSH水平在≥ 12mg/kg剂量范围继续呈现显著增加 (P <0 .0 1) ,睾丸匀浆中T在2 .4mg/kg剂量组则降低 (P <0 .0 5 )。ACP活性在染毒 15d时 2 .4mg/kg剂量组表现为升高 (P <0 .0 5 ) ,继续染毒至 30d时 6 0mg/kg剂量组则显著减低 (P <0 .0 5 ) ;γ GT活性则始终随染毒剂量的增加而降低 (P <0 .0 5 )。Spr与染毒剂量有明显的剂量依赖关系 ,在≥ 12mg/kg剂量范围显著减少 (P <0 .0 1)。 结论 :Fen对雄性大鼠有明显的生殖毒性 ,可影响其血清及睾丸性激素水平和酶活性 。
Objectives: To evaluate the impairment of fenvalerate on reproductive and endocrine systems and clarify the mechanism of action. Methods: Different doses of fenvalerate (0?2.4?12?60 mg/kg) were orally treated to the adult male SD rats for 15 days and 30 days, respectively. The levels of serum follicle stimulating hormone(FSH), luteinizing hormone (LH), testosterone(T) and testis homogenate T were determined by radioimmunoassay(RIA). Besides, the activity of testicular marked enzymes such as acid phosphatases(ACP) and gamma glutamyl transpeptidase(γ GT) were examined,and sperm head counts were measured to explain the changes of daily sperm production(Spr). Results: In fifteen days, serum FSH levels markedly increased in rats exposed to fenvalerate of ≤12 mg/kg groups( P <0.01) and serum levels of LH increased in 12 mg/kg group( P <0.01). In addition,T levels in testis homogenates decreased after treated with the doses of ≥12 mg/kg groups compared with the control group( P <0.01). In thirty days, serum contents of FSH were significantly elevated in the doses of ≥12 mg/kg groups( P <0.01) and homogenate levels of T were diminished in the low dose group(2.4 mg/kg)( P <0.05). Activity of ACP increased in 12 mg/kg group after fifteen days( P <0.05) and was restrained in the high dose group(60 mg/kg) in thirty days( P <0.05), but the contents of γ GT were arrested with different doses dependently in the testis( P <0.05). Fenvalerate caused dose dependent reduction in sperm head counts and daily sperm production, which markedly reduced at the doses of ≥12 mg/kg groups( P <0.01). Conclusions: Fenvalerate has obvious reproductive toxicity on male rats and can change their serum and testis homogenate levels of sex hormone or activity of testicular marked enzymes,which may be correlated with the impairment of Sertoli cell and spermatogenic epithelium.
出处
《中华男科学杂志》
CAS
CSCD
2002年第1期18-21,共4页
National Journal of Andrology
基金
国家"九五"医学科技攻关项目 (96 90 6 0 4 11)
国家攀登项目子课题 (2 0 0 0 5 0 4)
