摘要
目的 探讨低硒状态下大鼠心肌硫氧还蛋白还原酶（TR）的活性和表达变化,分析体内硒元素含量改变对硫氧还蛋白还原酶活性的影响及其可能的机制。方法 用低硒饲料喂养断乳2周的Wistar大鼠14周,分别采用生物化学方法、Western blot和Northern blot杂交检测心肌组织中TR的活性改变及TR蛋白、基因表达变化。结果 低硒组大鼠心肌TR活性降低至对照组的63.8％,差异有非常显著意义、蛋白杂交的峰面积密度扫描结果显示,常硒组为3 438,低硒组为 2 067。Northern blot结果显示低硒组与对照组无明显差异。结论 低硒能够降低心肌TR的活性和蛋白表达水平,但并不影响此酶的基因表达。提示低硒导致心肌TR活性降低可能是由于TR蛋白合成过程中SeCys插入减少所致。
Objective To study the changes of activity and expression of thioredoxin reductase (TR) in the selenium-deficient rats and to analyse the effect of selenium content in organism on thioredoxin reductase activity and possible mechanism. Methods Wistar rats were fed on selenium-deficient diets for 14 weeks. The changes of thioredoxin reductase activity, protein expression and gene expression were detected by biochemical methods, Western blot and Northern blot. Results Selenium deficiency caused a decrease in activity of the enzyme to 63. 8% of control in cardiomyocytes, and a decrease in thioredoxin reductase protein levels, but gene expression was not changed. Conclusions Selenium deficiency decreases activity and protein levels of thioredoxin reductase in cardiomyocytes. These changes might be caused by the decrease of selenium (in the form of SeCys) insertion during protein synthesis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2002年第2期97-99,共3页
Chinese Jouranl of Endemiology
