摘要
以健康妇女人工流产的刮宫物为原料,经50%乙醇溶液抽提、醋酸钠50%乙醇缓冲溶液分级沉淀、硫酸铵分段盐析,最后用DEAE—纤维素柱层析纯化.终产品放免活性为3500IU/mg,产品总回收率为40～50mg‰.终产品经PAG圆盘电泳分析,只出现1条电泳区带,并与纯度相近的尿HCG产品的电泳特性一致.
A simple and effective procedure was developed for the extraction and purification of HCG from the materials of induced abortion. The extraction product A with 0.3 mol/L NaAc-HAc buffer (pH4. 8) in 50% ethanol. The specific activities of HGG in each extraction were stepped up to about 100, 500 IU/mg and the yield percentages were about 90% , 80% , respectively. The purification procedure included fractionated preciptation with (NH4)2SO4 salting out and chromatography on DEAE-cellulose DE52. The specific activities of HCG in each purification were stepped up to about 1600, 3500 IU/mg and the yield percentages were about 80% and 85%, respectively. The purified preparation showed only one band of HCG by polyacry-lamide gel electrophoresis. The electropherogram of the final product was similar to that of the relevant purified product of urine HCG.
关键词
人工流产物
绒毛膜
促性腺激素
human chorionic gonadatropin (HCG)
extraction
purification
materials of induced abortion
