摘要
首先应用血凝抑制试验(HI)和对流免疫电泳交叉试验,对从我国分离的貂肠炎病毒(MEV)、豹细小病毒(LPV)、犬细小病毒(CPV)和貉细小病毒(RPV)进行了血清学比较研究,发现这4种病毒在血清学上关系密切.然后从感染病毒48h的猫肾传代细胞中分别提取、纯化这4种病毒的中间复制型DNA(RF-DNA),以λ—DNA—Hind Ⅲ片段为分子量标记,确定4种病毒的RF—DNA分子大小均在5kb左右.再应用限制性内切酶Hac Ⅲ和Hind Ⅲ分别完全酶切4种病毒的RF—DNA.结果表明,应用Hind Ⅲ酶切,4种细小病毒的RF—DNA都产生3个酶解片段;应用Hac Ⅲ酶切,MEV产生3个酶解片段,其余3种病毒产生4个相同的酶解片段,从而可将MEV与其余3种病毒明显地区别开.Hac Ⅲ酶切MEV和CPV的结果与国外报道的“MEV产生5个片段,CPV产生6个片段”不同.
Mink enteritis virus(MEV), leopard parvovirus(LPV), canine parvovirus (CPV) and racoon parvovirus(RPV) were compared by serological tests and restriction enzyme analysis.The haemagg lutination-inhibition (HI) test does not distinguish the four parvoviruses. Using counter- immunoelectrophoresis (CIE), the viral antigens have approximately the same precipitating lines with the homologous sera as with heterologous ones. The double- stranded replicative form of viral DNAs(RF- DNAs)has about 5kb in size. After complete digestion with restriction endonuclease Hind Ⅲ, each of four RF- DNAs produces three fragments in same length.After digestion with Hae Ⅲ, MEV has three restrictive fragments, the other three viruses have four fragments in same length. Using Hae Ⅲ,RF- DNA fragments of MEV and CPV are obviously different from those reported in foreign countries.
