摘要
本研究以pAT153质粒为载体,构建了小肠结肠耶氏菌毒性质粒(pVYE)经限制性内切酶Bam HI和PstI消化产生的DNA片段的基因文库.实验克隆了8株(pYBI～8)pVYE的BamHI片段和6株(pYPI～6)pVYE的PstI片段的重组子.其中pYB4、pYB7、pYB8重组质粒中插入的pVYE—Bam HIDNA片段的分子量分别为8.7、3.8、20kb;pYP3、pYP5、pYP6重组质粒中插入的pVYE-Pst I DNA片段的分子量分别为4.5、3.0、7.0kb.本实验结果为进一步筛选和制备特异性基因探针奠定了基础.
Gene Libraries of virulence plasmid DNA fragments of Y. enterocolitica (pVYE) digested by restriction enzyme Bam HI or Pst I were constructed with the cloning vector pAT 153 plasmid Of the recombinant clones, 8(pYB 1 - 8) fragments cut by Bam HI and 6( pYP 1 - 6) fragments by Pst I were introduced into E. coli HB 101.The molecular weights of pYB 4 , pYB 7,pYB 8 are 8.7kb, 3.8kb and 20kb and those of pYP 3,pYP 5,pYP 6 4.5 kb, 3.0 kb and 6.8 kb, respectively. The results of this study would establish the fundamental for screen and preparation of the specific gene probe of virulent Y. enterocolitica.
基金
青年科学基金支持项目
关键词
小肠结肠
耶氏菌
毒性质粒
基因
Yersinia enterocolitica, virulence plasmid
gene library, gene probe
