摘要
对杭州转塘患“抖抖病”的中华绒螯蟹进行了病原的分离和鉴定 ,从病蟹体内分离到两株病原菌 ,均属革兰氏阴性杆菌 ,单极生鞭毛 ,运动 ,兼性厌氧 ,发酵葡萄糖产酸不产气 ,氧化酶、七叶苷、过氧化氢酶阳性 ,能还原硝酸盐等 ,其形态特征及生理生化反应与豚鼠气单胞菌基本相同 ,故鉴定为豚鼠气单胞菌 .人工注射感染健康蟹后 ,均在 3d内死亡 ,死亡率为 10 0 %,证实豚鼠气单胞菌为中华绒螯蟹的致病菌 .药敏试验结果表明 ,此病原菌对链霉素、卡那霉素、新霉素、环丙沙星、氟哌酸、氯霉素、庆大霉素、妥布霉素、氟嗪酸、壮观霉素、复达欣、头孢呋肟、菌必治、萘啶酸、呋喃妥因等药物高度敏感 .
Two strains of bacteria were isolated from haemolymph, liver, ascites and muscle of the diseased Eriocheir sinensis, which were cultured in the ponds in Zhuantang, Hangzhou. Both of the isolates were Gram-negative rods, motile by means of single polar flagella, and were facultatively anaerobic. Glucoce was catabolized with production of acid and no gas. Oxidase, Esculin, Catalase were positive, and nitrite was prodused from nitrate. Morpholohic, physiological and biochemical characteristics of the present isolates were the same as those of Aeromonas caviae, therefore, the pathogens isolated from the diseased Eriocheir sinensis were identified as Aeromonas caviae. The mortality of artificial infection was 100% , which ascertained that the bacterial isolates were the pathogens of the Eriocheir sinensis. The pathogens were highly sensitive to streptomycin, kanamycin, neomycin, ciprofloxacin, norfloxacin, chloramphenical, gentamicin, tobramycin, ofloxacin, ceftazidime, cefuroxime, ceftriaxone, nalidixic acid, nitrofurantoin.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2001年第6期677-681,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家"九五"攻关资助项目 (96 - 0 15 - 0 1- 0 10 )
杭州市科委资助项目 (9912 1B0 7)
