摘要
Conditions for biotransformation and purification of FUR were investigated.The result showed that when the cell concentration of E.aerogenes was 10%(w/v),the temperature and pH were 7.8 and 60℃ respectively,59.7% UR was converted to FUR.It also demonstrated that the ideal procedure for the purification of FUR is silica gel column chromatography by two elution systems (S 1∶CHCl 3∶CH 3OH∶H 2O 61∶13∶1,and S 2;CH 3COOCH 2CH 3∶CH 3OH∶CH 3COOH∶H 2O 12∶3∶3∶2)with the purity and yield of 98.0% and 86.0% respectively.
Conditions for biotransformation and purification of FUR were investigated.The result showed that when the cell concentration of E.aerogenes was 10%(w/v),the temperature and pH were 7.8 and 60℃ respectively,59.7% UR was converted to FUR.It also demonstrated that the ideal procedure for the purification of FUR is silica gel column chromatography by two elution systems (S 1∶CHCl 3∶CH 3OH∶H 2O 61∶13∶1,and S 2;CH 3COOCH 2CH 3∶CH 3OH∶CH 3COOH∶H 2O 12∶3∶3∶2)with the purity and yield of 98.0% and 86.0% respectively.
出处
《微生物学报》
CAS
CSCD
北大核心
2002年第1期117-120,共4页
Acta Microbiologica Sinica
基金
国家医药管理局资助项目
关键词
5-氟尿苷
微生物转化
分离纯化
FUR, Biotransformation,Isolation and purification
