摘要
为了研究呼吸性酸碱失衡时内髓集合管 (IMCD)细胞病理生理学改变 ,分别以 3%CO2 和 10 %CO2 模拟慢性呼碱和慢性呼酸家兔肾脏IMCD细胞培养模型 ,对照组用 5 %CO2 。荧光探针法测定IMCD细胞H+ /K+ 交换功能 ,原位杂交和RT PCR检测IMCD细胞cH K ATP酶mRNA表达。结果显示 ,呼酸组H+ /K+ 交换显著高于对照组 (P <0 0 5 ) ,呼酸组cH K ATP酶mRNA原位杂交积分光密度和RT PCR积分光密度比值均非常显著地高于对照组 (P <0 0 1) ;而这三项指标 ,对照组与呼碱组之间差异均无显著性意义 (P >0 0 5 )。提示慢性呼酸显著增加IMCD细胞H+ /K+ 交换和cH K ATP酶mRNA表达 ,慢性呼碱有抑制和下调趋势 ,但差异无显著性意义 。
To study the cellular pathophysiological change in kidney IMCD cell under respiratory acid base disorders. 3% or 10% CO 2 was used to initiate chronic respiratory alkalosis(ALG) or chronic respiratory acidosis(ACG) of cultured rabbit IMCD cell. 5% CO 2 was used in control group(CG). Fluorescent probe was used to measure H + /K + exchange function of these cells. In situ hybridization and RT PCR were used to measure cH K ATPase mRNA expression of these cells.The results showed that H + /K + exchange in ACG was significantly higher than that in CG ( P <0 05), the accumulation light density ratio of cH K ATPase mRNA in situ hybridization and the accumulation light density ratio of cH K ATPase mRNA with RT PCR in ACG were siginificantly higher than those in CG( P <0 01).There was no siginificant difference among the three indexes between CG and ALG ( P >0 05). It is suggested that chronic respiratory acidosis could induce an increase in H + /K + exchange function and cH K ATPase mRNA expression. However, chronic respiratory alkalosis could induce decreases in H + /K + exchange function and cH K ATPase mRNA expression with no statistical significance, the reason of which calls for further study.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第3期225-227,共3页
Medical Journal of Chinese People's Liberation Army
