摘要
目的 :胰岛素样生长因子 (IGF 1)对动脉粥样硬化的形成和发展及骨骼组织的矿化均起重要作用 ,鉴于动脉钙化广泛存在于动脉粥样硬化病变中而且与骨骼矿化过程的相似性 ,本研究试图研究IGF 1在动脉钙化中的作用。方法 :体外培养牛主动脉平滑肌细胞 ,建立血管钙化模型 ,在培养液中加入不同浓度的IGF 1,观察细胞钙沉积变化 ,以及与之密切相关的细胞碱性磷酸酶活性和骨钙素分泌情况。结果 :10 - 9mol L的IGF 1培养 10d后 ,普通对照培养细胞的钙沉积无明显变化 ,钙化培养细胞的钙沉积增加了 11 82 % (P >0 0 5 ) ,在此过程中细胞内碱性磷酸酶活性增加了 16 8 7% ,骨钙素分泌增加了 5 8 3% ;增加IGF 1的浓度至 10 - 8mol L和 10 - 7mol L ,细胞的钙盐沉积进一步增加 ,分别提高了 2 2 74 % (P <0 0 5 )和 4 0 7% (P <0 0 1)。结论 :IGF 1可呈剂量依赖性的促进钙化血管细胞的钙质沉积 。
Objective:Accumulated evidence suggested that vascular calcification share many characteristics with the minaralization of bone IGF 1 can enhance the minaralization of osteoblastic cell in bone and is also one of the important growth factors in blood vessel So it is rational to study its role in the vascular calcification Methods: β glycerin phosphate disodium salt was used to induce cultured bovine vessel smooth muscle cell to calcify which serves as a vascular calcifying model in vitro 10 -9 mol/L IGF 1 were add to the culture media After 10 days culturing, the deposition of calcium, alkaline phosphatase activity and osteocalcin concentration was quantified Results: After 10 days of culture, 10 -9 mol/L IGF 1 increased the calcium deposition of calcified BVSMC by 11 82%( P >0 05),this processes is time dependent The cellular ALP activity was dramatically increased with the highest in the 8 day with the greatest increase of 168 7% The osteocalcin concentration of cultured media syntheses by BVSMCs also elevated 58 3% Change the concentration of IGF 1 to 10 -8 mol/L and 10 -7 mol/L, the calcium deposition of BVSMCs increased by 22 74% ( P <0 05)and 40 7% ( P <0 01)respectively Conclusion:IGF 1 increased calcified BVSMCs' calcium deposition This may through the stimulating the cellular synthesis of ALP and osteocalcin which indicate that it promote the calcified BVSMCs' changing to osteoblastic cell
出处
《军医进修学院学报》
CAS
2002年第1期14-17,共4页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金资助项目 (98G0 3 3 9770 869)
