摘要
探讨碱性成纤维细胞生长因子 (b FGF)在肾癌中表达的意义和其在肾癌微血管形成中的作用及与肾癌生物学特性的关系。采用斑点杂交技术测定 2 0例肾癌患者癌组织、癌旁和正常肾组织b FGF表达情况 ;用免疫组化技术测定 35例肾癌患者癌组织、癌旁和正常肾组织b FGF表达和肿瘤微血管密度 (MVD)。结果显示 ,肾癌组织中b FGF表达明显高于癌旁和周围正常肾组织 (P <0 0 1)。b FGF表达升高与肿瘤分期、分级密切相关 ;肾细胞癌组织中MVD与b FGF表达呈明显正相关。提示b FGF是肾细胞癌组织中重要的血管生长因子之一 ,b FGF表达和MVD与肾细胞癌的预后有关。
The aim of this study was to investigate the expression of basic fibroblast growth factor (b FGF) and its relationship with tumor microvasogenesis and biological behavior of human renal carcinoma. Gene expression of b FGF was assayed in 20 cases of renal carcinoma tissue using RNA dot blot hybridization. Meanwhile, the protein expression of b FGF and microvasular density (MVD) was assayed in 35 cases of renal carcinoma tissue with immunohistochemical assay. It was found that the expression of b FGF was significantly higher in renal cancer tissue than in normal renal tissue or normal tissue adjacent to the tumor, There was significant correlation between b FGF expression in cancer tissue and clinical stages as well as clinical grades.Similar results were also observed between MVD and b FGF expression in renal cancer tissue. It seemed that b FGF played an important role in angiogenesis in renal carcinoma. Taken together, the results suggest that MVD and the expression of b FGF in renal cancer tissue might be related to the prognosis of renal carcinoma patients.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第3期246-247,共2页
Medical Journal of Chinese People's Liberation Army
