摘要
本文采用血管紧张素 (Ang )所诱导的乳牛基底动脉平滑肌细胞 (BAVSMCS)增殖模型 ,以 MTT法观察甲壳胺对 BAVSMCS增殖的影响 ,[3 H]Td R掺入法观察甲壳胺对乳牛基底动脉平滑肌细胞 DNA的合成 ,还观察了甲壳胺对乳牛基底动脉平滑肌细胞总蛋白的影响。结果表明 :甲壳胺对 Ang 所诱导的BAVSMCS增殖有浓度和时间依赖性抑制作用 ;可浓度依赖性抑制 Ang 所诱导 BAVSMCS的 DNA合成增加作用 ;还可抑制 Ang 所诱导 BAVSMCS蛋白合成增多作用。提示甲壳胺可抑制 Ang 所诱导的 BAVSM-CS增殖作用。
The bovine basilar artery vascular smooth muscle cells(BAVSMC S) were isolated and cultured.The effects of chitosan and enalaprilat on the proliferation of BAVSMC S induced by angiotensin Ⅱ(AngⅡ) were evaluated with MTT method,the DNA synthesis in BAVSMC S was measured by thymidine incorporation and total protein of BAVSMC S was detected by coomassie brilliant blue method.The results showed that chitosan can inhibit the proliferation of BAVSMC S induced by AngⅡ in a time and concentration dependent manner,and depress thymidine incorporation of BAVSMC S induced by AngⅡ in a concentration dependent manner chitosan can reduce the content of protein in BAVSMC S induced by AngⅡ.These findings suggested that chitosan is capable of producing an inhibitory effect on proliferation of bovine basilar artery vascular smooth muscle cells induced by AngⅡ .
出处
《中国海洋药物》
CAS
CSCD
2002年第1期11-14,共4页
Chinese Journal of Marine Drugs
