摘要
目的 建立一种检测血清中可溶性CD13 7(sCD13 7)的方法 ,探讨sCD13 7对类风湿性关节炎 (RA)的意义。方法 建立链霉亲合素 酶联免疫吸附实验 (SA ELISA)以检测RA活动期患者 ( 3 0例 )、RA缓解期患者 ( 3 0例 )、正常对照者 ( 3 0例 )的血清sCD13 7水平 ,并用免疫速率散射比浊法检测血清CRP、RF水平。结果 SA ELISA有较好的线性、灵敏度、特异性、重复性和准确性 ;RA活动期组的血清sCD13 7阳性率和水平显著高于RA缓解期组和正常对照组 (P <0 0 1) ,且与CRP、RF水平间存在明显正相关 (P <0 0 1)。结论 SA ELISA能可靠检测sCD13 7;sCD13 7可能成为反映RA活动性的指标之一。
Objective To establish a method for the measurement of soluble CD137(sCD137),measure sCD137 levels in the sera from patients with rheumatoid arthritis(RA) and estimate its significance. Methods Serum sCD137 and other immunological parameters,e.g.,CRP,RF in 30 patients with active RA,30 patients with inactive RA and 30 healthy controls were measured by streptavidin ELISA(SA ELISA) established and immunological rate nephelometry respectively.Results SA ELISA had good linearity,sensitivity,specificity,reproducibility and accuracy.The positive percentage and level of serum sCD137 in active RA group were significantly higer than that in inactive RA group and control group( P <0 01).There was significantly positive correlations between sCD137 levels and CRP,RF in active RA group( P <0 01). Conclusion sCD137 can be reliably measured by SA ELISA,sCD137 might be a valuable parameter for reflecting the activity of RA.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2002年第1期13-14,共2页
Chinese Journal of Clinical Laboratory Science
