摘要
用邻苯二甲醛 (OPA) -3_巯基丙酸 (3_MPA)柱前衍生高效液相色谱法测定了采自大亚湾近岸海域的一个长60cm的沉积物柱样W0中15种游离氨基酸 (FAA)的含量 ;结果表明 ,W0 中FAA总量在0.82～2.69mmol/kg之间 ,15种FAA以及FAA总量均随深度而以指数曲线降低 ;对氨基酸组成的分析表明 ,天冬氨酸、甘氨酸、组氨酸和苯丙氨酸的摩尔分数随深度而上升 ,苏氨酸、精氨酸、缬氨酸和异亮氨酸的摩尔分数随深度而下降 ,其他氨基酸的摩尔分数随深度无显著变化。甘氨酸、丙氨酸、天冬氨酸、谷氨酸、丝氨酸是W0中含量最丰富的氨基酸 ,其摩尔分数依次为28.1%、16.0 %、14.7 %、10.6%和7.2 %。
free amino acids(FAA) were measured in a sediment core collected in Daya Bay of South China Sea by high performance liquid chromatography and pre _ column derivatization using o _ phthaldialdehyde and 3 _ mercaptopropionic acid as derivating agents. The results showed that contents of total FAA in the sediment core were in the range from 0.82 to 2.69 mmol/kg(dry weight) and all 15 FAA contents decreased with depth exponentially. The mole fractions of aspartic acid, glycine, histidine and phenylalanine increased with depth, while mole fractions of threonine, arginine, valine and isoleucine decreased with the depth and other amino acids had no noticeable changes. Glycine, alanine, aspartic acid, glutamic acid and serine were the most abundant FAA in the sediment with the mole fractions being 28.1%, 16.0%, 14.7%, 10.6% and 7.2%, respectively.
出处
《分析测试学报》
CAS
CSCD
北大核心
2002年第2期4-7,共4页
Journal of Instrumental Analysis
基金
广东省自然科学基金资助项目 (20000088)
中国科学院大亚湾海洋生物综合实验站基金资助项目 (P -9905)
广东省环保科技开发基金资助项目 (粤环 -97-23)
