摘要
目的 :探讨胃癌组织P53、P1 6 、K ras基因的协同突变情况 ,揭示P53、P1 6 、K ras基因突变与胃癌发生的关系。方法 :应用聚合酶链反应 (PCR)与单链构象多态性分析法 (SSCP)对 6 0例经病理切片诊断为胃癌组织和 2 0例正常胃粘膜组织进行P53、P1 6 、K ras基因突变分析。结果 :6 0例胃癌粘膜组织中 ,P53基因发生突变 5 3例 ,占 88 3% ;P1 6 基因发生突变 49例 ,占 81 7% ;K ras基因发生突变 5 6例 ,占 93 3% ;男女间突变率比较差异无显著性 (P >0 0 5 ) ;而 2 0例正常胃粘膜组织无P53、P1 6 、K ras基因突变发生。结论 :P53、P1 6 、K ras基因突变是胃癌中常见的分子事件 ,用PCR—SSCP法联合检测胃粘膜组织P53、P1 6 、K ras基因的突变情况将有助于诊断胃癌 。
Objective:To study P 53 ?P 16 ?K ras gene mutation in gastric cancer mucus tissue,and to disclose the relationship between gastric cancer and P 53 ?P 16 ?K ras gene mutation.Methods:PCR SSCP assay was used to analiyze P53?P16?K ras gene mutation in gastric mucus tissue pre matastasis of 60 cases and 20 controls.Result:P 53 gene mutation was found in 60 cases(88 3%),the gene mutation of P 16 were 49 cases(81 7%),and K ras were 56 cases(93 3%).Also we found that no significant difference between sex of P 53 ;P 16 ?K ras mutation(P>0 05);while the gene mutation was not found in 20 controls.Conclusion:P 53 ?P 16 ?K ras gene mutation in gastric mucus tissue is a usual molecule events,and PCR SSCP is a useful method to help to understand the biological behavir of gastric cancer.
出处
《广西医学》
CAS
2001年第5期1017-1019,共3页
Guangxi Medical Journal
