摘要
1983年,Lohka和Masui首先报道,用精子染色质与卵提取物一起温育,可以组装成具有典型结构的细胞核。这种核不仅具有合成DNA的能力,而且还能进入M期。同年,
Previous studies have shown that the typical nucleus can be assembled around the naked lambda DNA in cell free Xenopus egg extracts. However, it is not very clear if the nucleus may be formed around the other exogenous naked DNA in the cell-free system. In our experiment, we used the purified adenovirus DNA to reconstitute the nucleus in the nuclear reconstitution extracts and found that typical nuclei can also be assembled around the added DNA. Generally, 2.500 new nuclei are formed in one egg's extract. The most assembled nuclei are 10-15 μm in diameter, and possess a double-membranized nuclear envelope with typical nuclear pore complex structures and chromatin-like materials. In control samples of no added DNA, no nucleus is formed. So, we get a conclusion that the purified adenovirus DNA can also be used to induce the nuclear reconstitution in cell-free extracts of Xenopus eggs.
出处
《实验生物学报》
CSCD
1991年第3期287-291,共5页
Acta Biologiae Experimentalis Sinica
基金
自然科学基金
国家教委博士生点基金
关键词
爪蟾
卵提取物
非细胞体系
核重构
Xenopus laevis. Egg extract. Cell free system. Nuclear reconstitution.
