摘要
目的 :探讨CBCD34 + 细胞扩增的最佳HGF组合及移植时机。方法 :CBCD34 + 细胞培养于无血清培养液中 ,分别加入下列HGF ,A组 :对照组 (无HGF) ;B组 :SCF ,IL 3,IL 6 ;C组 :SCF ,IL 3,IL 6 ,G CSF ,Epo ;D组 :SCF ,IL 3,IL 6 ,TPO ,Flt3 L ;E组 :SCF ,IL 3,IL 6 ,TPO ,Flt3 L ,G CSF ,Epo。培养 2 2d。对不同培养时间的细胞进行NC数量及系特异性CD动态观察。结果 :同对照组相比 ,扩增组NC和CD34 + 细胞均有不同程度的扩增。NC和CD34 + 细胞扩增高峰分别在第 10天和第6天。E组的扩增效果最佳。CD检测显示 ,各组CD34 + 及CD34 + CD38 细胞的比例逐渐减少 ,但B ,D 2组的CD34 + CD38 细胞的比例在第 6天有一过性回升 ,CD14+ ,CD13+ ,CD6 1+ 及Gly A+ 细胞的比例则逐渐升高 ,D组CD34 + 及CD34 + CD38 细胞的比例明显地高于E组 (P <0 .0 1) ,CD3+ 和CD19+ 细胞的比例在扩增中呈下降趋势。结论 :HSPC分化程度与HGF组合及培养时间有关。就细胞总数而言 ,E组HGF组合最佳 ,但就HSPC含量来说 ,以D组为优。第 1周扩增产物中HSPC含量较高 ,移植时间以扩增的第 6～ 10天为宜。
Objective: To explore the optimal combination of HGF for expanding CD34 + cells and the suitable time for transplantation.Methods: CB CD34 + cells were cultured for 22 days in medium containing following combination of HGFs: Group A (Containing no HGFs as control), group B (SCF,IL-3,IL-6), group C (SCF,IL-3,IL-6,G-CSF,Epo),group D (SCF,IL-3,IL-6,TPO,Flt3-L), group E (SCF,IL-3,IL-6,TPO,Flt3-L,G-CSF,Epo). At every intervals, the cells were collected and assayed for CD. Results: NC and CD34 + cells were expanded significantly in all expansion group, the peak of number of NC is on day 10 and that of CD34 + cells is on day 6. The highest level of NC is in group E, and that of CD34 + and CD34 + CD38 - cells is in group D. The content of CD34 + and CD34 + CD38 - cells decreased gradually. Interestingly, the content of CD34 + CD38 - cells in group B and D increased slightly on day 6 and that of CD14 + ,CD13 + ,CD61 + Gly-A + cells elevated increasingly correspondingly. However, the content of CD3 + and CD19 + cells decreased during expansion. Conclusions: Differentiation of CD34 + cells related to combination of HGF and time of ex vivo expansion , SCF combines with IL-3,IL-6,TPO,Flt3-L,G-CSF and Epo is optimal combination regarding to the number of NC, but in view of the content of HSPC, the combination containing SCF,IL-3,IL-6,TPO and Flt3-L is suitable for expansion of HSPC. The content of HSPC reached the highest level in first week, so the suitable time for transplantation of expanded cells is from day 6 to day 10 of expansion.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2001年第4期248-252,共5页
Chinese Journal of Cancer Biotherapy
基金
国家"九五"医学科技攻关课题基金 (编号 96 90 6 0 1 17)
国家自然科学基金 (批准号 39870 77)
上海血液学研究所胡应洲基金部分资助
