摘要
目的：克隆ＩκＢ－α基因，构建ＩκＢ－α的真核表达载体。方法：ＲＴ－ＰＣＲ扩增ＩκＢ－α的ｃＤＮＡ，然后将其克隆入真核表达载体ｐＳｈｕｔｔｌｅ，并转入大肠杆菌ＪＭ１０９。结果：通过ＰＣＲ和重组质粒酶切分析等方法，筛选出重组阳性克隆。结论：此重组质粒可用于真核表达等进一步研究。
Objective:To clone IκB-α gene and construct its eu karyotic expression vector. Methods:The cDNA encoding IκB-α was am plif ied from total RNA by RT-PCR to introduce xba I site at 5′ end kpn I site at 3 ′ end, and then cloned into the vector pShuttle. Results:The recombin ant clones were analyzed by the methods of PCR and restriction enzyme digestion. Conclusion:These recombinant clones may be used in further studies.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第2期130-131,149,共3页
Journal of Nanjing Medical University(Natural Sciences)
