摘要
目的:探讨咳喘宁胶囊对实验性慢性支气管炎治疗作用的内在机制。方法:采用改良烟熏法复制大鼠慢性支气管炎模型,实验分组为模型组、咳高组、咳低组、桂龙组,并设不加处理的正常对照组。以高、低剂量咳喘宁和桂龙咳喘宁进行干预,用放免法测定各组大鼠血清、肺组织及支气管肺泡灌洗液(BALF)中血栓素B_2(TXB_2)和6-酮-前列腺素F_(1α)(6-keto-PGF_(1α))的含量。结果:模型组大鼠血清、肺组织及BALF中TXB_2和6-keto-PGF_(1α)及其比值均较正常组明显升高(P<0.01);与模型组相比较,咳高组和桂龙组血清、肺组织及BALF中TXB_2和6-keto-PGF_(1α)含量及其比值均有明显降低(P<0.05或P<0.01),而咳低组无明显差异;咳高组血清、肺组织及BALF中TXB_2和6-keto-PGF_(1α)含量显著低于桂龙组(P<0.05或P<0.01)。结论:咳喘宁胶囊可通过降低TXB_2和6-keto-PGF_(1α)含量发挥治疗作用。
To make research on the internal mechanism of treating experimental chronic bronchitis with Kechuanning Capsule. We duplicated the animal model of chronic bronchitis with refined smoking-fumigated method, the rats were allocated to model group, high dose group, low dose group , Guilong group and normal control group that didn t treated. Made intervention with high and low dose of Kechuanning as well as Guilong Kechuanning (another medicine of the similar kind) and determined the TXB2 &6-keto-PGF1α contents in the blood serum, lung tissue and BALF of each group with radiom-munoassay. The TXB2 &6-keto-PGF1α content as well as its radio in the blood serum, lung tissue and BALF of the model group were significantly improved than the normal group ( P < 0.01) . Compared with the model group, the TXB2 &6-ke-to-PGF1α content as well as its ratio in the blood serum, lung tissue and BALF of the high dose group and Guilong group were significantly reduced (P <0.05 or P < 0.01) ; while the low dose group hadn't significant difference.The TXB2 &6-keto-PGF1α content as well as radio in the blood, lung tissue and BALF of the high dose group were significantly lower than that of the Guilong group ( P < 0.05 or P < 0.01). Kechuanning capsules could produce therapeutical effect by decrease the TXB2 &6-keto-PGF1αcontent as well as its ratio.
出处
《中国医药学报》
CSCD
2002年第1期23-26,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
河北省科技厅资助项目(97276157B)
