摘要
目的 探讨稳定、高效、低毒的基因转导载体系统在基础研究和临床应用中的重要意义。方法 采用文献回顾的方法并结合当前实验研究 ,对肝细胞基因转导载体系统的研究和应用进展加以综述。结果 病毒载体系统和非病毒载体系统均可实现目的基因对肝细胞的有效转导 ,转导效率因各载体系统生物学特性不同而各异。结论 肝细胞是基因治疗有效的靶细胞 ,多种与肝脏相关的遗传性疾病、恶性肿瘤、感染性疾病等均可实现靶基因的有效转导 。
Objective To investigate the stable, high effective and low toxicity gene transfer vectors' basics in research and clinical application.Methods Current status of hepatocyte directed gene transfer vectors were reviewed by history document and contemporary experimental advances analysis.Results Viral and nonviral vector systems could both transduce target genes to liver effectively with various transduction rates based on their corresponding biological characteristics.Conclusion Hepatocyte is the effective target for gene therapy of liver related genetic, neoplastic and infectious diseases, although the security needs to be further evaluated.
出处
《中国普外基础与临床杂志》
CAS
2002年第1期58-60,共3页
Chinese Journal of Bases and Clinics In General Surgery
