摘要
目的观察血小板源性生长因子(PDGF)两亚基(A,B)和α和β两种受体在增生性瘢痕形成机制中的作用。方法用免疫组化方法和常规病理技术检测这四种蛋白在8例增生性瘢痕和8例正常皮肤中的表达变化规律。结果PDGF-A在正常皮肤与增生性瘢痕中的阳性细胞率分别为(15.2±5.6)%和(18.9±4.1)%,而PDGF-B分别为(22.4±7.4)%和(25.5±6.5)%。从正常皮肤到增生性瘢痕,两亚基含量虽有升高趋势,但差异不明显(P>0.05)。PDGFR-α在正常皮肤与增生性瘢痕中的阳性率分别(36.7±4.3)%和(44.2±5.8)%,而PDGFR-β则分别为(15.3±4.8)%和(22.9±5.2)%,两种蛋白在瘢痕中的表达阳性率都显著高于正常皮肤(P<0.05)。结论增生性瘢痕形成可能与PDGFR-α和PDGFR-β蛋白高表达有关,而PDGF在增生性瘢痕发生中的作用,还需进一步探讨。
Objective To explore the effect of platelet derived growth factor two subunits(A,B )and their receptors(PDGFR-αand PDGFR-β)protein expression on the formation of hypertrophic scar.Methods The expression intensity and distribution of PDGF-A,PDGF-B,PDGFR-αand PDGFR-βwere examinated with immunohistoch emistry and pathological methods in8cases of hypertrophic scars and8cases of normal skins which were obt ained from patients in our hospital.Results The positive expression rates of PDGF-A and PDGF-B in normal skins were(15.2±5.6)%and(22.4±7.4)%respectively,were(18.9±4.1)%and(25.5±6.5)%in hypertrophic scars re-spectively,which showed no significant difference between normal skin s and hypertrophic scars(P>0.05).The positive expression rates of PDGFR-αand PDGFR-βwere(44.2±5.8)%and(22.9±5.2)%respectively in hypertrophic scars,significantly higher than that in normal skins(36.7±4.3)%and(15.3±4.8)%respectively,P<0.05.Conclusion The increment of PDGFR-αand PDGFR-βcontents in hypertrophic scars versus in norm al skins might be one of the mechanism s controlling scar formation,in whi ch the role of PDGF need to be deeply studied.
出处
《中国临床康复》
CSCD
2002年第4期514-515,共2页
Chinese Journal of Clinical Rehabilitation
基金
国家重大基础研究规划资助项目(G1999054204)
国家杰出青年科学基金(39525024)
全军九五医学科研基金重点课题(98Z090)
