摘要
目的研究苦参碱(matrine,Mat)对兔耳增生性瘢痕成纤维细胞增殖活性的影响。方法利用兔耳腹侧面增生性瘢痕模型,采取免疫组织化学法观察增殖细胞核抗原的表达(proliferationcellnuclearantigen,PCNA)及嗜银核仁组织区染色法(AgNORs)统计NORs颗粒数,对比研究苦参碱在治疗兔耳增生性瘢痕过程中对成纤维细胞增殖活性的影响。结果局部注射苦参碱后,兔耳腹侧面增生性瘢痕中的成纤维细胞增生活性受到明显抑制,PCNA表达明显减弱,AgNORs记数明显减少。用药12周后对照组/苦参碱组PCNA标记指数和AgNORs分别为0.87/0.35,2.31/1.07(P<0.05)。结论苦参碱明显抑制兔耳增生性瘢痕成纤维细胞增生活性,对兔耳腹侧面增生性瘢痕有明显的治疗作用。
Objective To investigate the effectiveness of matrine on hypertrophic scarring mo del in rabbit ears.Method With comparison between the controls and the matrine groups which were injected the matrine in the model of hypertrophic scarring in rabbits,to study the changes of proliferative activity o f the fibroblasts derived from hypertrophic scarring .Results Matrine could inhibit the fibroblasts prolifer-ative activity including the expression of proliferation cell nuclear a ntigen and AgNORs which were weaker t han the controls from 0.87,2.31to0.35,1.07(P<0.05)respectively.Conclusion Matrine is able to inhibit the prolif erative activity of fibroblasts derived hypertrophic scarring tissue of rabbit ears and has effecte d on hypertrophic scarring signific antly.
出处
《中国临床康复》
CSCD
2002年第4期510-511,共2页
Chinese Journal of Clinical Rehabilitation
关键词
苦参碱
成纤维细胞增殖活性
兔耳增生性瘢痕
创伤愈合
matrine
proliferative activity of fibroblasts
hypertrophic scarrin g of rabbit ear scar model
