摘要
目的探讨毫米波局部照射后,除局部效应外。对动物神经系统、内分泌器官、免疫及其他内脏、生殖器官、胚胎发育及子代有无远位效应。方法选用不同频率、不同功率密度的毫米波辐照小鼠、大鼠、豚鼠,并于照后不同时间,应用先进的技术方法,(光镜、电镜、DNA定量分析、组化、免疫组化、细胞培养、高效液相(HPLC)、RIA、RBA、TAS、畸形学分析、精神行为学分析等)从功能、形态、代谢等不同角度对实验动物进行观察分析。结果系列实验研究证明,在毫米波局部照射后确可引起远位组织、器官发生许多变化(骨髓中多核巨细胞核固缩,睾丸精管中出现多核巨细胞,肝细胞中糖原含量有变化,肝中cAMP、cGMP有起伏,G-6-P酶活性有变化,血液细胞成分有变化)。小鼠孕早期(1～2d)局部受照射,可见胚卵细胞表面一些酶活性有改变,导致胚卵着床减少;小鼠孕后期(5～15d)局部皮肤受照射,其胚胎虽未发生畸形,但其生下之后一代,学习记忆能力有所降低。结论毫米波局部照射确有远位效应。本项研究为临床毫米波的应用和防护标准的制定奠定了理论基础。
Objective To investigate the distant bioeffec ts after local irradiation of millim eter wave.such as influence on nervo us system,endocrine,immune,reprodictive sy stem,and another viscera,on embryo、offspring of the animals.Methods We have used the most developed techniques and methods,such as microscopy,electron microscopy,DNA quantitative analysis,histochemistry,immunohistochemisty,cell culture,high pressure liquid chrom atography(HPLC),TAS,RIA,RBA,teratogical analysis,psychophysiological analysis,etc.From the view of function,morphology and metabolis m,we carried out a comprehensive stu dy in this project.Results After local radiation ,obvious chan ges were found in distant tissues and organs(pycnosis of megacaryocytes in bone marrow,occurrence of multinuclear g iant cells in seminiferous canalicu li in testis,changes of glucogen conte nt in liver cell,dynamic alteration in content of cAMP,cGMP,glucose-6-phosphatase and quantity changes of blood composition).When mice were radiated at early sta ge of gestation(1～2d of gestation),morphologocal alteration of the ce lls on the surface of blastomere,changes of its enzymatic activity and decline of blastomere implantation rate were fo und.When mice were radiated at the later stage of gestation(6～15d of gestation),though malformation was not found i n posterity,but capacity of learnin g and memory of the posterity was lower than normal.Conclusion The millimeter wave local irradiati on has certainly distant bioeffects,this results has proved an important theoretical basis for the clinical use of mm -microwave and formulation of its safty standard.
出处
《中国临床康复》
CSCD
2002年第4期492-493,513,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金(84)科基金准字413
毫米波生物效应
毫米波非热效应省科研基金(3880252)
辐射对优生的影响及其防护(87K-44)
毫米波辐照孕鼠对其胚胎及生后仔鼠的影响(92KY-GIT)
