摘要
目的 观察毛木耳多糖 (APSP)体外对小鼠腹腔巨噬细胞 (Mφ)蛋白激酶C(PKC)活性的影响。 方法 采用反相离子对高效液相色谱法测定PKC活力。结果 APSP (80mg·L-1)能引起小鼠腹腔Mφ中PKC活性明显升高 ,2 0min达峰值 ,1.5h恢复到基础水平 ,并可引起Mφ中PKC发生质膜转位。
Aim To investigate the effect of Auricularia Polytricha Sacc polysaccharide(APSP) on PKC activity in murine peritoneal macrophages.Methods A new ion-pair reversed-phase high liquid chromatography method was used to determinate the activity of protein kinase C in cells.Results APSP-A(80mg·L -1 ) induced activation and translocation of PKC.The peak time was 20 minutes,the activity of PKC came back the basic level at 1.5 hours.Conclusion The immunopotentiating effect of Auricularia Polytricha Sacc polysaccharide may be associated with its activation of PKC in murine peritoneal macrophages.
出处
《解放军药学学报》
CAS
2002年第1期19-20,共2页
Pharmaceutical Journal of Chinese People's Liberation Army
