摘要
为探讨内皮衍生因子 (NO/ET)与慢性胃溃疡形成的关系 ,采用大鼠醋酸性胃溃疡模型 ,分别以 L-精氨酸(L- Arg)和 NG-硝基 - L-精氨酸 (L- NNA)腹腔内注射 ,测定溃疡指数 ,以硝酸还原酶法和放射免疫技术 ,分别检测血清 NO和血浆 ET- 1含量。结果显示 :1L- Arg组溃疡指数显著低于模型组和 L- NNA组 (P<0 .0 1) ,L- NNA组溃疡指数显著高于模型组 (P<0 .0 1)。 2 L- Arg组血清 NO含量显著高于模型组及对照组 (P<0 .0 1) ,L- NNA组血清 NO含量显著低于以上 3组 (P<0 .0 1)。 3L- Arg组血浆 ET- 1水平显著低于模型组和对照组 (P<0 .0 1) ,L- NNA组血浆ET- 1水平显著高于以上各组 (P<0 .0 1)。提示内皮衍生因子的失衡可能是慢性胃溃疡形成的重要原因。
To investigate the relationship between endothelium-derived factors--nitric oxide (NO) and endothelin (ET) and chronic gastric ulcer, the rat model of gastric ulcer was induced by acetic acid. The index of ulcer (UI) was measured by using a vernier, and the level of serum NO and plasma ET-1 determined by the methods of nitric acid reductase and radioimmunoassay respectively. The results were as follows: (1) UI in L-Arg group was significantly lower than in the model group and L-NNA treated group (P<0.01), While UI in the L-NNA group was obviously higher than in the model group (P<0.01); (2) The level of serum NO in the L-Arg group was dramatically higher than in the model group and control group (P<0.01), while the level of NO in the L-NNA group was lower than in other three groups (P<0.01); (3) The level of plasma ET-1 in the L-Arg group was obviously lower than in the model group and control group (P<0.01), while the level of plasma ET-1 in the L-NNA group was dramatically higher than in other three groups (P<0.01). It was suggested that the uneven level of NO and ET is a key factor in the course of development of chronic gastric ulcer.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2002年第1期75-77,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家中医药管理局科研基金资助项目 (95 B15 1)
关键词
内皮衍生因子
消化性溃疡
一氧化氮
内皮素
endothelium-derived factors
peptic ulcer
nitric oxide
endothelin-1
