摘要
目的 观察ＰＤＴＣ对创伤后肺组织ＴＮＦα、ＩＬ－８含量及其ｍＲＮＡ表达的影响。方法８０只大鼠分为创伤组（Ｔ组）、ＰＤＴＣ预处理组（Ｐ组）及对照组（Ｃ组），利用多功能小型生物撞击机致伤，观测伤后各组０．５、１、２、４、８ ｈ肺组织ＴＮＦα、ＩＬ－８及其ｍＲＮＡ变化。结果 致伤后ＴＮＦα、ＩＬ－８含量及其ｍＲＮＡ表达明显升高，ＴＮＦα在伤后１ｈ达到峰值，ＩＬ－８在伤后４ｈ达到峰值；ＰＤＴＣ组 ＴＮＦα、ＩＬ－８含量及其ｍＲＮＡ表达显著低于创伤组（Ｐ＜０．０１）。结论ＰＤＴＣ可以通过抑制ＴＮＦα、ＩＬ－８ ｍＲＮＡ表达，减少致炎细胞因子的释放，减轻创伤后全身及肺组织的炎症损害。
Objective To investgate the effect of pyrrolidine dithiocarbamate (PDTC) on TNFα, IL-8 content and their mRNA expression in traumatic acute lung injury in rats. Methods A total of 80 Wistar rats were divided into traumatic group (T), PDTC pretreatment group (P) and control groups. The traumatic acute lung injury was obtained by multi-funtion minitype bio-impact machine with driving pressure of 350 kPa on the right superior lateral chest wall of rats. The contents of TNFα, IL-8 and the expression of TNFα, IL-8 mRNA were determined by ELISA or RT-PCR respectively. Results The TNFα, IL-8 content and the expression of TNFα, IL-8 mRNA in traumatic group were markedly increased, the peak of TNDα, IL-8 were at 1 h, 4 h after trauma respectively. The TNFα, IL-8 contents and the expression of TNFα, IL-8 mRNA were markedly lowerr in PDTC group(P < 0.01) than that in group T. Conclusion PDTC can alleviate the traumatic acute lung injury by inhibiting the expression of TNFα and IL-8 mRNA.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第8期957-959,共3页
Journal of Third Military Medical University
基金
国家重点基础研究发展规划项目(G1999054203)
高等院校全国优秀博士论文作者专项基金(199948)
