摘要
目的 对临床分离菌株进行鉴定，分析耐利福平结核分支杆菌是否在人群中传播。方法 采用一对随机引物（Ｐ１５’－ＣＣＧＧＧＧＣＣＧＧＴＴＣ；Ｐ２ ５’－ＣＣＧＣＣＣＡＣＣＧＡＣ），对从重庆地区肺结核病人痰标本中分离到的１００株耐利福平结核分支杆菌的 ＤＮＡ进行随机ＰＣＲ扩增，根据扩增产物的ＤＮＡ电泳指纹进行分型。结果１００株耐利福平结核分枝杆菌的ＤＮＡ指纹可分为６种类型，以Ⅱ、Ⅰ和Ⅲ型为主，它们分别占４０％、３１％和２５％；这３型菌株有１／４是从初治结核病人的痰标本中分离，而在２０－３９岁年龄段则有１／３是从初治结核病人的痰标本中分离。结论 随机扩增ＤＮＡ指纹是一种简便、敏感、重复性好的菌株分型方法，可用于耐药性结核病流行监测。重庆地区结核病的流行很可能与耐利福平结核分支杆菌的传播有关。
Objective To determine whether Mycobacterium tuberculosis resisting to rifampin transmits among people. Methods The finger- prints of genomic DNA from M tuberculosis strains resisting to rifampin, isolated from sputum specimens of 100 pulmonary tuberculosis patients, were obtained by random amplified polymorphic DNA (RAPD) fingerprinting with primer 1 (5' -CCGGGGCGGTTC) and primer 2 (5'-CCGCCGAC- CGAC). Results Six types of DNA fingerprints were classified; the main types were II, I and III and they were 40%, 31 %, and 25%, respec- tively. Approximately 24% of type I, II, and III strains were isolated from the patients who had received inifial anti-tuberculosis treatment; approxi- mately 31% of the 3 type strains were isolated from the first treated young patients (20-39 year old). Conclusion The RAPD fingerprinting is an useful method to type M tuberculosis strains; the prevalence of tuberculosis resisting to rifampin in Chongqing city may be primarily due to the trans- mission of type II, I and III strains.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第8期942-944,共3页
Journal of Third Military Medical University
基金
重庆市医学重点资助项目(99-1002)
重庆市科委攻关资助项目(2000年)
关键词
结核分支杆菌
利福平
耐药
DNA指纹
结核病
Mycobacterium tuberculosis
rifampin
drug resistance
DNA fingerprinting
