摘要
以典型的籼 /粳交 (窄叶青 8号 /京系 17) DH群体为材料 ,采用视频显微扫描技术和计算机图形分析相结合的技术手段 ,从横切面、侧面和腹面 3个层面对 12 7个 DH株系的垩白大小进行了立体分析 ;并结合该群体业已构建的染色体图谱检测控制垩白的数量性状位点 (QTL)。对横切面的分析检测到 3个 QTL s,分别位于第 8、11和 12染色体上的 RZ6 17～G2 132、ATT42 B～ RG98和 CT46 2～ RG5 74标记区间内。对侧面的分析也检测到 3个 QTL s,其 L OD值的最高峰位点与横切面分析完全一致。对腹面的分析检测到 3个 QTL s,分别位于第 8、11和 12染色体上的 GA376～ CT195、RZ6 38～ RG30 4和CT46 2～ RG5 74标记区间内 ,其加性效应来源与横切面和腹面分析的一致。 3个切面的有关 QTL的 LOD值的分布峰区和最高峰位点的一致或相互覆盖也表明 ,应用视频显微扫描技术分析稻米垩白任一切面的数据均可定位控制垩白大小的 QTL。
A doubled haploid population, derived from anther culture of F 1 hybrid between a typical indica cv. Zhaiyeqing 8 and a japonica cv. Jingxi 17, has been used in this study. The chalkiness sizes of transverse section, flank section and belly section, were investigated in 127 DH lines with video microscopy and computer image analysis technique. The quantitative trait loci (QTLs) for the size of chalkiness were analysed. A total of nine QTLs were detected in five intervals of three chromosomes, including three QTLs for transverse section, three QTLs for flank section and three QTLs for belly section. The directions of additive effect of QTLs for three sections were identical, and the intervals and peak of LOD on three chromosomes were overlapping, some of them were uniform. The application value of video microscopy and computer image analysis technique in rice chalkiness analysis were discussed.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2002年第1期11-14,共4页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目 ( 39970 471)
浙江省自然科学基金资助项目 ( 30 0 2 2 1)
浙江省自然科学基金重点资助项目
