摘要
考虑到蛋白质在结晶过程中溶液和晶体之间的动态平衡 ,蛋白质在溶液中的聚合状态决定了晶体堆积的最小单元 .通过动态光散射实验以及计算机模拟 ,对大鼠金属硫蛋白亚型 和兔肝金属硫蛋白亚型 和 的聚合物形式进行了比较 ,可以确定大鼠金属硫蛋白亚型 在结晶条件下的缓冲液中应该以四聚体而不是二聚体形式存在 .通过考察晶体的模型也可以发现 ,晶体的确可以通过四聚体的形式堆积得到 。
Considering that the conformational units should be the same in solution as in crystals during crystallization equilibrium, the minimal crystal packing unit is determined by the style of protein aggregations in solution. Based on the comparison of MT Ⅱ from rat liver and MT Ⅰ and MT Ⅱ from rabbit, it could be proven that in NaH 2PO 4 Na 2HPO 4 buffer, the aggregate of MT Ⅱ from rat liver would be tetramer, not dimer. From the analysis of the crystal packing model, it could be naturally deduced that the crystals were obtained from the packing of tetramers; moreover, the crystal packing could be comprehended more deeply by using the tetramer packing model.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第2期271-274,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 9992 5 90 2 )资助
