摘要
目的 :检测卵巢上皮性肿瘤中c erbB2、c myc和k ras12 ,13在基因和蛋白质水平的改变 ,探讨促使卵巢癌发生的癌基因谱成员。方法 :免疫组化检测c erbB2、c myc的蛋白质表达 ,原位杂交和Southern杂交检测c erbB2、c myc的基因改变 ,PCR检测k ras12 ,13的点突变。结果 :卵巢囊腺癌中c erbB2、c myc的基因扩增率和蛋白质过表达率均显著高于卵巢囊腺瘤 ,卵巢粘液性肿瘤的k ras12 ,13突变率高于浆液性肿瘤。结论 :在卵巢癌的发生中c erbB2和c myc起一定作用 ,c erbB2起重要作用。两者均可为卵巢癌的癌基因谱成员 ;k ras12 ,13点突变在卵巢浆液性肿瘤中作用不明显。在粘液性肿瘤中 ,k ras点突变除在细胞过度增殖中起作用外 ,还能促进细胞的粘液性分化。
Objective:To detect gene alterations and protein expressions of c-erbB2,c-myc and k-ras 12,13 in ovarian epithelial neoplasms and to discuss the oncogene map that contributes to the formation of ovarian carcinoma.Methods: Gene alterations and protein expressions of c-erbB2,c-myc and k-ras 12,13 in ovarian epithelial neoplasms were determined by immunohistochemistry,DNA hybridization and PCR. Results:The rates of gene amplification and protein overexpressions of c-erbB2 and c-myc were significantly higher in ovarian carcinoma than those in ovarian adenoma.The k-ras12,13 mutation rate of ovarian mucinous adenocarcinoma was higher than that of the ovary serous neoplasms.Conclusions:Oncogenes c-erbB2 and c-myc take part in the formation of ovarian carcinoma .C-erbB2 plays an important role during the process.They both can be the member of oncogene map in ovary adenocarcinoma.The mutation of k-ras12,13 may play role in ovarian serous neoplasms.In ovarian mucinous adenocarcinoma,k-ras take part in the cell hyperplasia as well as mucinous differentiation.
出处
《现代妇产科进展》
CSCD
2001年第5期346-348,共3页
Progress in Obstetrics and Gynecology
基金
山东省自然科学基金资助项目 (95 6 2 0 8)
关键词
卵巢肿瘤
癌基因
蛋白质表达
Ovarian neoplasms
Oncogene
Protein expression
