摘要
利用DNA结合免疫分析证明,编码人U_1和U_2 snRNA基因的5′端区域含有与SV40 T抗原特异结合的序列。SV40 T抗原与U_2基因的亲和力大于U_1基因。DNasel足印(footprinting)分析取得与DNA结合免疫分析一致的结果。U_1和U_2基因的5′端区域含有能被T抗原所保护的,免于DNasel降解的序列。这两个基因的两条链上都含有约30bp长的DNA被T抗原所保护。U_1基因被保护的区域在-11bp—-42bp之间,而U_2基因被保护区域是在-58bp—-90bp之间。
Using DNA immunoassays we have shown that the 5'-flanking region of the genes encoding human Ul and U2 snRNA contain regions that are able to bind tightly and specifically with the SV40 large tumor antigen (T Ag). The affinity of T Ag to U2 gene is greater than that to Ul gene. DNase I footprinting showed that 5'-flanking regions of both Ul and U2 contain sequences that were specifically protected against digestion by T Ag. About 30nt regions on either strand of DNA in human Ul and U2 genes were protected by T Ag. These regions were located at -11 to -42 bp on Ul gene and -58 to -90 bp on U2 gene.
关键词
SV40T抗原
snRNA基因
免疫
SV40 T antigen
U1
U2 snRNA gene
DNA immunoassay
Dnase I footprinting
