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大鼠睾丸AQP7mRNA与蛋白质的表达及定位研究 被引量：3

Study on expression and localization of AQP7 mRNA and protein in rat testis
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摘要 目的 :探讨 AQP7在大鼠睾丸的基因与蛋白质表达及定位。方法 :取 4周和 1 2周 Wistar大鼠睾丸 ,采用 RT- PCR、Western blot检测 AQP7m RNA和蛋白质表达 ,免疫组化检测进行其定位研究。结果 :大鼠睾丸有 AQP7m RNA和蛋白质表达 ,定位于减数分裂后的精子细胞和精子膜上 ,管周细胞也有 AQP7的阳性染色。结论 Objective: To localize AQP7 mRNA and protein expression in 4 and 12 week rat testis. Methods: Use the testes of Wistar male rats of 4 and 12 weeks old for RT PCR, Western blot and immunohistochemical localization of AQP7. Results: There were expression of AQP7 mRNA and protein in rat testes.It was localized in the membrane of meiotic spermatids, sperms and periductular cells. Conclusion : AQP7 is possibly related to spermatogenesis and the function of seminiferous tubule.
作者 刘庆双 李大男 赵丹 赵雪俭 赵丽娟 杨宝学
机构地区 吉林大学基础医学院生殖病生研究室 长春医学高等专科学校 美国加州大学
出处 《吉林大学学报（医学版）》 CAS CSCD 北大核心 2002年第1期14-16,共3页 Journal of Jilin University：Medicine Edition
关键词 AQP7 睾丸 蛋白质 表达 定位 AQP7 rat testis protein expression and localization
分类号 R339.21 [医药卫生—人体生理学] Q593.9 [生物学—生物化学]
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参考文献3

  • 1IshibashiK, Kuwahara M, Kageyama Y, et al. Cloning and functional expression of a second new aquaaporin abundantly expressed in testis [J]. Biochem Biophy Res Commur, 1997,237: 714-718.
  • 2Suzuki TF, Ishibashi K, Yuasa S. Immunohistochemital localization of a water channel, aquaporin (AQP7), in the rat testis [J]. Cell Tissue Res, 1999, 295 (2): 279-285.
  • 3Ishibashi K, Kuwahara M, Gu Y, et al. Cloning and functional expression of a new water channel abundantly expressed in the testis permeable to water, glycerol, and utea [J]. J Biol Chem, 1997, 272 (33): 20782-20786.

同被引文献13

  • 1Yang B. The human aquaporin gene family[J]. Current Genomics,2000,1(1):91-102.
  • 2Yang B,Gillespie A,Carlson EJ,et al. Neonatal mortality in an aquaporin-2 knock-in mouse model of recessive nephrogenic diabetes insipidus[J]. J Biol Chem,2001,276(4):2775-2779.
  • 3Ishibashi K,Kuwahara M,Gu Y,et al. Cloning and functional expression of a new water channel abundantly expressed in the testis permeable to water,glycerol,and urea[J]. J Biol Chern,1997,272(33): 20782-20786.
  • 4Morise H,Shimomura O,Johnson FH,et al. Intermolecular energy transfer in the bioluminescent system of Aequorea[J]. Biochemistry,1974,13(12):2656-2662.
  • 5Prasher DC,Eckenrode VK,Ward WW,et al. Primary structure of the Aequorea victoria green-fluorescent protein[J]. Gene,1992,111(2):229-233.
  • 6Chalfie M,Tu Y,Euskirchen G,et al. Green fluorescent protein as a marker for gene expression[J]. Science,1994,263(5148):802-805.
  • 7J.萨姆布鲁克,EF.弗里奇,T.曼尼阿蒂斯著.分子克隆实验指南[M].第2版,北京:科学出版社,1998.343-361.
  • 8Carissa MK,Bellamkonda KK. Aquaporins: the membrane water channels of the biological world[J]. Biologist,2003,50(2):81-86.
  • 9Calamita G,Mazzone A,Bizzoca A,et al. Possible involvement of aquaporin-7 and -8 in rat testis development and spermatogenesis[J]. Biochem Biophys Res Commun,2001,288(3):619-625.
  • 10王宏丽,张薪薪.水-甘油通道蛋白AQP7与高脂肪饮食诱导的小鼠肥胖机制的研究[J].中国实验诊断学,2009,13(10):1337-1338. 被引量：4

引证文献3

二级引证文献11

吉林大学学报（医学版）
2002年 第1期

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