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5α-还原酶m RNA在Wistar大鼠附睾内的随龄表达研究

Study on the expression of 5α-reductase of increasing postnatal age in Wistar rat′s epididymis
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摘要 目的 :观察附睾内 5 α-还原酶 ( 型 ) m RNA的表达是否随年龄增长而改变。方法 :采用斑点杂交的方法 ,用 c DNA探针检测附睾中 型酶 m RNA的表达量。结果 :在附睾头、体、尾部 ,5 α-还原酶 ( 型 ) m RNA的表达量于青春期前 ( 2 1 d)、青春期 ( 4 2 d)、成龄 ( 91 d)差异均无显著性。结论 :年龄增长对附睾内 5α-还原酶 ( 型 ) m Objective:To investigate if the expression of 5α reductase type Ⅱ mRNA changes with increasing postnatal age in epididymis.Methods:The expression level of 5α reductase type Ⅱ mRNA in epididymis was detected in dot blot using cDNA probe.Results:5α reductase mRNA level in the caput,corpus and cauda of epididymis was no different in prepuberty(21 day),puberty(42 day) and adult(91 day) rat.Conclusion:the increasing postnatal age does not affect the expression of 5α reductase in epididymis.
出处 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2002年第1期6-8,共3页 Journal of Jilin University:Medicine Edition
基金 国家自然科学基金资助课题 (39870 313)
关键词 5Α-还原酶 附睾 基因表达 年龄 reductase epididymis gene expression age
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