摘要
目的 :通过研究不同剂量 X射线全身照射后小鼠脾淋巴细胞运铁蛋白 (Tf R)表达的变化 ,探讨电离辐射后 Tf R对免疫功能的作用。方法 :应用直接免疫荧光抗体和流式细胞仪检测 Tf R表达的变化。结果 :75m Gy X射线全身照射后 2 4 h和 72 h,脾脏中 Tf R阳性表达细胞数显著增加 ;但 1～ 6Gy X射线全身照射后 2 4 h,脾脏中 Tf R阳性表达细胞数显著减少。同时检测的 IL- 2活性显示出平行变化。结论 :低剂量电离辐射时 Tf R增强免疫功能 ,而在高剂量电离辐射时 Tf R则抑制免疫功能。 Tf R表达的变化可能是因为电离辐射使 IL-
Objective:To explore effect of TfR on immune function after ionizing by investigating changes in TfR expression on splenic lymphocytes of mice after whole body irradiation (WBI) with different dose X rays. Methods: Direct immunoflurescence antibodies and flow cytometry were used to examine the changes of TfR expression. Results: The cell number of TfR positive expression in spleens increased significantly at 24 hours and 72 hours after WBI with 75 mGy X rays but the cell number of TfR positive expression in spleens decreased significantly at 24 hours after WBI with 1~6 Gy X rays. The activity of IL 2,meanwhile, demonstrated a parallel change. Conclusion: These results suggest that the TfR enhances immune function in low dose ionizing radiation but suppresses immune function in high dose. The change of TfR expression may be due to the change of IL 2 activity caused by ionizing radiation.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2002年第1期1-5,共5页
Journal of Jilin University：Medicine Edition
