摘要
目的 :探讨定量PCR检测血清HBV -DNA与血清HBV标志物之间的关系及其临床意义。方法 :35 2例血清标本运用荧光定量PCR检测血清HBV -DNA及放射免疫法 (ELISA)检测血清HBV标志物。结果 :经荧光定量PCR检测 94例HBsAg、HBeAg、HBcAb均阳性的标本 ,其血清HBV -DNA全部阳性 ,DNA测值范围 4 .8× 10 5～ 2 .8× 10 8copy/ml;81例HBsAg、HBeAb、HBcAb均阳性的标本 ,其血清HBV -DNA4 3例阳性(阳性率为 5 3% ) ,DNA测值范围 1.0× 10 4～ 1.4× 10 7copy/ml;39例HBsAg、HBcAb阳性的标本 ,其血清HBV-DNA2 4例阳性 (阳性率为 6 1% ) ,DNA测值范围 3.5× 10 3 ～ 1.6× 10 7copy/ml;10 0例HBVM全阴性的标本 ,血清HBV -DNA全部阴性。结论 :荧光定量PCR检测血清HBV -DNA准确灵敏 ,可反映乙肝患者病程变化 ,对于乙肝临床诊断、治疗方案的选择、疗效观察及预后判断有极其重要的作用。
Objective:To investigate the relationship between serum HBV-DNA and serum HBV markers (HBVM) and its clinical significance.Method:HBVM and HBV-DNA were tested in 352 serum samples by enzyme linked immunosorbent assay (ELISA) and quantitative fluorescence PCR technique respectively.Results:Serum HBV-DNA positive rates of the patients with HBsAg,HBeAg and HBc Ab triplex positive was 100% (94/94),the DNA detective values were that from 4.8×10 5 to 2.8×10 8copy/ml.The positive rate was 55% (43/83)for positive sera with HBsAg,HBe Ab and HBcAb,the patients DNA levels were that from 1.0×10 4to 1.4×10 7copy/ml.The sera HBV-DNA positive rate of the patients who had positive both the HBsAg and HBcAb was 61% (24/39),their the DNA range from 3.5×10 5 to 1.6×10 7 copy /ml.However,100 Samples which had all negative HBVM were that of negative results of sera HBV-DNA assay. Conclusions:The quantitative fluorescence PCR determination of serum HBV-DNA could reflect the course of HBV infection,so it has an important value in the clinical diagnosis,selection of therapeutic scheme,efficacy evaluation and prognosis for the patients with HBV infection.
出处
《中国现代医学杂志》
CAS
CSCD
2002年第4期51-52,54,共3页
China Journal of Modern Medicine
