摘要
目的 研究异丙酚在脊髓水平是否有镇痛作用。方法 SD大鼠 15只 ,随机分为三组 :对照组、芬太尼组、异丙酚组 ,分别腹腔注射 0 9%生理盐水 ,0 1mg/kg芬太尼或 10 0mg/kg异丙酚 ,2min后 ,向大鼠右侧后肢跖部皮下注射福尔马林 ,1h后 ,用Fos免疫组织化学方法观察脊髓内c fos基因的表达。结果 福尔马林仅诱导Fos在同侧脊髓表达 ,预注芬太尼或异丙酚明显抑制Fos的表达 ,Fos免疫反应阳性神经元 (FLIN)数量分别降低了 5 7 8%、36 3% (P <0 0 1) ,且芬太尼的抑制作用强于异丙酚 (P <0 0 1)。结论 睡眠剂量的异丙酚在脊髓水平有镇痛作用 。
Objective To investigate whether spinal cord is involved in the analgesic effect of propofol.Methods Fifteen adult SD rats of both sexes weighing 195～223g were randomly divided into three group of five animals each: control group received normal saline intraperitoneally(ip), fentanyl group received fentanyl 0 1mg/kg and propofol group propofol 100mg/kg ip 2 min later 4% formalin 150μl was injected subcutaneously into the planta region of right hindpaw 1h after formalin injection animals of all three groups were anesthetized with pentobarbital sodium ip After induction of anesthesia chest was open and 100 ml of normal saline was infused via left ventricle then followed by 4% formalin infusion for fixation of tissue, 90 min later spinal cord, L 3 5 sect, was removed for determination of c fos expression in spinal cord using fos immunohistochemistry technique Results In control group in less than 10 s after formalin intraplantar injection the animals were agitated, restless, lame and paw licking In fentanyl group and propofol group the righting reflex was suppressed for (19 4±7 8) min and (7 2±1 5)min and no pain response was seen during this period When righting reflex recovered the pain response was much lighter than that in the control group Formalin stimulation induced c fos expression was seen only in the ipsilateral spinal cord Both fentanyl and propofol significantly suppressed c fos expression evoked by formalin stimulation The number of fos like immunoreactivity neurons(FLIN) decreased by 57 8% and 36 3% respectively(P<0 01) Suppression of c fos expression was greater under fentanyl than propoful(P<0 01) Conclusions Propofol has analgesic effect at hypnotic dose and spinal cord is involved in its analgesic effect The analgesic effect of propofol is smaller than that of fentanyl
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2001年第7期423-425,共3页
Chinese Journal of Anesthesiology
