摘要
为了探讨脑组织移植的机制,本实验对大鼠进行了实验形态学研究。用谷氨酸单钠(MSG)损毁大鼠弓状核,用15～18天鼠胚下丘脑组织移入第三脑室,存活3～5个月。然后对正常(N)组,损毁(D)组及损毁后移植(T)组动物的下丘脑和垂体前叶进行光镜、电镜和免疫细胞化学观察,对血清的睾酮进行生化测定。结果表明:MSG能选择性地损毁弓状核内约80%的神经元。第三脑室内的移植物呈团块状,能存活并与宿主脑组织紧密连接,两者之间存在交界面或直接融合。荧光显微镜下可见移植物内有发黄绿色荧光的儿茶酚胺能神经细胞,免疫细胞化学显示移植区内存在TH阳性神经细胞,后者为梭形或多角形。电镜观察表明:移植区内有神经细胞、神经纤维、各种胶质细胞和毛细血管,并且存在轴-树和轴-轴突触。免疫电镜还显示有TH阳性神经元胞体和轴突末梢,后者可作为突触前成分与TH阴性结构形成突触。此外,免疫组化显示T组垂体前叶的GH和FSH(FSH在雄性动物能促进曲细精管发育和精子生成)细胞较D组明显增多,放免分析表明T组血清中睾酮含量也比D组明显升高。本研究还证明接触脑脊液的神经元在脑组织移植中有重要作用。
In order to approach the mechanism of the brain tissue transplantation, experimental monphological studies were taken in rats. The arcuate nuclei of the hypothalamus were lesioned by monosodium glutamate. The fetal (15-18 days) hypothalamic fragments were implanted trite the third cerebral ventricle of the lesioned rats. The animals were allowed to survive for 3-5 months.Then the hypothalamus and the anterior lobe of the hypophysis of the normal (N), lesioned (D) and transplanted (T) group were observed by light and electron microscopes after immunocytochemical treatment. The testosterone of blood serum in each group were biochemically analysed and compared. The results showed that monosodium glutamate selectively distroyed neurons in the arcuate nucleus (about 80%). The grafts survived as a tissue mass inserted in the cavity of the third ventricle and established close connection with the host hypothalamic tissue with or without contacting surface in between. In the grafts there were yellow-green fluorescence-bearing catecholaminergic neurons and TH immunoreactive neurons appearing as fusiform or polygonal shape. Electron microscopy demonstrated that grafts integrated well with host tissue and contained perikarya, nerve fibers, a variety of neuroglial cells, capillaries as well as axodendritic and axoaxonic synapses. Especially, there were TH-like positive cell bodies and axonal endings which formed synapses as presynaptic elements with TH negative postsynaptic structures. In addition, immunocytoehemistry identified that the GH and FSH cells in the anterior lobe of the hypophysis were much more in the T group than that in the D group. Radioimmunoassay showed that the testosterone concentration in blood serum were much higher in the T group than that in the D group. The survival of grafts in the third ventricle provided further evidence For the CSF-contacting neurons and their important role in the brain tissue transplantation.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
1991年第1期37-42,共6页
Chinese Journal of Neuroanatomy
基金
卫生部基金
关键词
下丘脑
脑
移植术
hypothalamus, the third cerebral ventricle, brain tissue iransplantation, the CSF-contacting neuron, rat
