摘要
目的 :采用EHECO15 7∶H7-EDL933株 ,体外染菌感染德国小蠊 (Blattelagermanica) ,观察其体内外感染和带菌消长情况。方法 :点滴法体外定量染菌 ,剂量为 1μl(菌悬液浓度为8.0× 10 5～ 1.9× 10 7(CFU /ml) ,并采用昆虫单虫单管方法饲养观察。结果 :蜚蠊经体外染菌 ,获得体内外带菌 ,体外带菌时间中位数为 ( 14 4 .0± 2 2 .17)h( 95 %CI :10 0 .5 5～ 187.45h) ;体内中位数为 ( 14 4± 2 5 .77)h( 95 %CI :93.48～ 194.5 2h) ;体内外带菌消长差异 ,无统计学意义( χ2 =1.46 ,P =0 .2 2 6 3)。德国小蠊最长带菌时间至少 2 16h( 9d)。结论 :蜚蠊不仅是EHEC -O15 7∶H7的贮存宿主和疾病传播的媒介 ,更重要的是潜在的人类感染的传染源。
Objective:The persistence of experimental Escherichia coli O157∶H7-EDL933 strain in cockroach (Blattela germanica) was studied.Methods:Cockroaches were externally contaminated by droplet 1 μl of EDL933 strain 8.0×10 5- 1.9×10 7 CFU/ml, after contaminating O157∶H7,each insect was individually placed into a sterilized glass tube (15 mm×150 mm) for 8 h-216 h.Results:The external and internal of cockroaches were infected with O157∶H7 by contaminating the pathogen.The median of external carriage O157∶H7 was 144.0 h±22.77 h(95CI:100.55 h-187.45 h); the median of internal was 144.0 h±22.77 h(95CI:93.48 h-194.52 h). The difference of persistence curve of Escheichia coli O157∶H7 carriage in external and internal of cockroaches was no statistically significance through Log Rank test (χ 2=1.46,P=0.226 3). Droplet experiments of O157∶H7 to cockroaches showed that the ingested bacteria were harbored in the intestine of cockroaches and continued to be excreted at least for 216 h(9 d).Conclusion:The result showed that the cockroaches were not simple reservoirs and mechanical vectors of EHEC O157∶H7, but an epidemiological potential source of O157∶H7 infection for humans.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2002年第1期34-36,共3页
Chinese Journal of Vector Biology and Control
基金
浙江省卫生厅基金项目 (98B0 81 )
