摘要
目的 :用淀粉电泳酯酶谱区别酯酶 ,检测淡色库蚊成蚊个体酯酶 ,并结合对幼虫的生物测试结果 ,探讨抗性生化机制及防制对策。方法 :幼虫的生物测试采用浸渍法 ,成虫的非特异性酯酶测试采用淀粉电泳法。结果 :淡色库蚊对氯氰菊酯抗性最高 ,是敏感品系的 12 .30倍 ;其后顺次为敌敌畏、高效氯氰、溴氰菊酯、残杀威、仲丁威 ,抗性倍数分别为 5 .6 1、5 .0 9、4.6 5、4.43、3.98,胺菊酯、二氯苯醚菊酯、EBT、三氯杀虫酯抗性不明显。淀粉电泳结果蚊虫个体中存在过量产生的非特异性酯酶Estβ11和Estα 2 /β2 ,且蚊虫的酯酶谱较为复杂。 结论 :淡色库蚊对氯氰菊酯产生了中等抗性 ;其次对敌敌畏、高效氯氰、溴氰菊酯产生低抗性 ,宜换用药物。酯酶染色结果 ,存在引起有机磷抗性增高活性酯酶 ,酯酶谱且呈多态分布 ,与生物测试结果相一致 ,所以应减少敌敌畏的使用 ,注意轮换用药。
Objective: To investigate the situation of the Hangzhou Culex pipiens pallens resistance,to differentiate esterase by using starch gel electrophoresis esterase flocculus and having biological test at the same time. Method:Dipping for instars and non-differential starch gel electrophoresis for imago. Results:The cypermethrin resistance was the highest one in six pesticides and it was 12.3 times of the sensitive strains.Dichlorphos,high-efficiency cypermethrin,deltamethrin,Propoxur,BPMC were respectively 5.61, 5.09, 4.65, 4.43, 3.98 times. The non-differential esterase Est β1 1 and Est α 2/β2 had also come into being. Conclusion:Hangzhou Culex pipiens pallens produced middle resistance to cypermethrin and low resistance to other five insecticides.Some active esterases which have higher resistance to Organic phosphorus are in existence.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2002年第1期22-23,共2页
Chinese Journal of Vector Biology and Control
关键词
淡色库蚊
抗性
酯酶活性
生物测试
杭州
Culex pipiens pallens
Resistance
Esterase
Biological test
