摘要
1987年上半年,我们研究出一种能快速产生香菇子实体的合成培养基。此化学合成培养基是由几种化学药品和一定数量燕麦粉制成的。在此培养基上接种香菇 Cr—02菌株和平菇(紫孢侧耳),进行二次短时间的低温刺激(13℃)。经过多次实验,香菇20天显蕾并出菇,培养35天时诱导显蕾率96.7—100%,成菇率平均79.3%,开伞率平均60%,平均每瓶出菇6.2个。此培养基接平菇无诱导作用。经过多次实验表明,刺激形成香菇子实体的主要诱导因子是燕麦粉。
The Lentinus edodes is the second of the most important cultivated mushroom in worldwide.Japan is the major country of production of L. edodes However,they failed to attempt i nduction L.edodes fruit on a chemically defined midium.This is due to the difficulty of fruiting edib- le fungi in pure culture on chemically defined media.The purpose of this study is to search a chemcally defined medium for the fruiting of L.edodes,and shorten its life cycle.So far,the fruiting of L.edodes on a synthetic medium is less reported. Ando(1974)reported the fruiting of L.edodes on a synthetic medium in a minimum of 57 day after inoculation.During the current study,Lea- tham(1983)led to the development of a defined medium rapid fruiting. Over 90% of the cultures produced button-stage mushroom within 45 day after inoculation.But,this medium consisted of more than twenty chemi- cal compounds.The production of this synthetic medium was very comp- lfcated to satisfy its conditions. We formulated a synthetic medium,which consisted of five chemical reagents supplemented with oat flour,and it is easy to be prepared.Some fruiting was produced at 22℃ on this medium within 20 day after inoc- ulation.Induction frequency of fruiting L.edodes was 100%.Over 714% of the cultures produced mature mllshroom with expanded caps. Primary research in this laboratory showed that the main factors of stimulating L.edodes fruiting was oat flour.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
1989年第3期35-40,共6页
Journal of Hebei Agricultural University
关键词
香菇
子实体
诱导
合成培养基
Lentinus edodes
Fruit-body
Iuduction
synthetic medium
