摘要
目的 研究快速、准确检测假肥大型肌营养不良 (DMD BMD)基因缺失的方法。方法 采用 9对引物的多重链式聚合酶反应 (mPCR)检测 16 9例DMD BMD基因缺失。结果 16 9例患者中 85例检测出基因缺失 ,总缺失率为 5 0 .3%。结论 9对引物的mPCR在DMD BMD基因缺失诊断中具有快速、准确、经济、实用等特点 ,便于推广。
Objective To investigate a quick and accurate method in detection of DMD/BMD gene deletions.Methods Detection of DMD/BMD gene deletions by mPCR of 9 pairs of primers was applied.Results 85 patients' gene was deleted in 169.The total rate of deletions was 50.3%.Conclusion mPCR of 9 pairs of primers was a quick accurate economic and practical method in detection of DMD/BMD gene deletions.
出处
《河北医药》
CAS
2001年第2期98-99,共2页
Hebei Medical Journal
