摘要
用聚合酶链反应(PCR)在3例乙型肝炎病毒(HBV)C基因阳性的原发性肝癌(HCC)组织中扩增和克隆了HBV C基因片段(部分前C区和全部C区),获得了重组质粒pHBC_1,pHBC_2,pHBC_3。将重组质粒中HBV C基因亚克隆至M_(13)DNA序列测定系统,用双脱氧法测定了HBV C基因片段的核苷酸序列。在2例HCC中,发现前C区第1898位核苷酸点突变,结果形成了终止密码。在3例HCC中,C区也发现有核苷酸点突变,其中部分可致编码氨基酸改变。
Using polymerase chain reaction, the HBV C gene fragments were amplified and cloned from 3 HBV C gene positive hepatocellular carcinomas. Reoombinant plasmids, pHBC1, pHBC2 and pHBC3 were obtained. The HBV C gene fragments from recombinant plsmidswere further suboloned into the M13 sequencing system and the fragments were sequenced by dideoxy chain termination. The point mutations of nuoleotide 1898 within the pre-0 region were identified in pHBC1 and pHBC2, which resulted in a stop oodon. In addition, some point mutations were found within C region in 3 recombinant plasmids and a few of them resulted in amino acid changes.
出处
《上海医科大学学报》
CSCD
1991年第2期95-99,共5页
Journal of Fudan University(Medical Science)
基金
国家科委资助课题
卫生部资助课题(75-62-01-07)
关键词
肝肿瘤
乙肝病毒
终止密码
hepatitis B virus
stop eodon
hepatocellular carcinoma
polymerase chain reaction
