摘要
目的检测正常妊娠妇女、习惯性流产患者外周血单个核细胞 (PBMC)内 T辅助细胞亚型 (Th1)细胞因子白细胞介素 - 2 (IL- 2 )、T辅助细胞 2亚型 (Th2 )细胞因子白细胞介素 10 (IL- 10 ) m RNA表达水平的差异。方法用半定量逆转录 -聚合酶链反应 (RT- PCR)的方法检测 10例正常非孕妇女、10例正常妊娠妇女、10例习惯性流产患者 PBMC内 IL- 2、IL- 10 m RNA的表达水平。结果同正常非孕对照相比 ,正常妊娠妇女 IL- 10 m RNA明显升高 ,习惯性流产患者 IL- 10 m RNA降低 ,IL- 2 m RNA明显升高 ;同正常妊娠妇女相比 ,习惯性流产患者 IL- 2 m RNA及 IL- 10 m RNA均明显降低。结论正常妊娠分泌 IL- 10升高 ,当孕妇分泌 IL- 10降低而分泌 IL- 2升高时 。
Objective To examine the expression of the cytokines of Th1(Interleukin 2) and Th2 (Interleukin 10) on the peripheral blood mononuclear cell (PBMC) of normal pregnant women and recurrent spontaneous abortion (RSA) patients. Methods The Interleukin 2 and Interleukin 10 mRNA were determined from PBMCs of 10 normal non pregnant women, 10 normal pregnant women and 10 patients with RSA by semi quantitative reverse transcriptional polymerase chain reaction (RT PCR) method. Results Comparing with the non pregnant women the Interleukin 10 mRNA was higher in normal pregnant women; the Interleukin 10 mRNA was lower, and the Interleukin 2 mRNA was higher in RSA women. And the Interleukin 10 mRNA was lower, while the Interleukin 2 mRNA was higher in RSA women compared with the normal pregnant women. Conclusion The results suggest that in normal pregnancy main secretion is Interleukin 10. It may cause RSA, when Interleukin 10 mRNA decreases while Interleukin 2 mRNA increases in pregnant women.
出处
《上海医学检验杂志》
北大核心
2001年第6期340-342,共3页
Shanghai Journal of Medical Laboratory Sciences
