摘要
目的 :观察脂多糖 (LPS)对大鼠肺微血管内皮细胞 (RPMVECs)G蛋白的影响及甲基强的松龙的干预作用。方法 :用流式细胞技术 (FCM )检测G蛋白亚型的变化。结果 :① 10 μg/mlLPS作用于RPMVECs 30和 90min后 ,Gsα蛋白水平较对照组显著下降。甲基强的松龙干预 30和 90min后 ,对LPS致RPMVECs膜Gsα蛋白水平的变化有显著抑制作用。② 10μg/mlLPS作用于RPMVECs 30和 90min后 ,Giα蛋白水平较对照组显著下降。甲基强的松龙干预 30和 90min后 ,对LPS致RPMVECs膜Giα蛋白水平的变化有显著抑制作用。结论 :①LPS诱导RPMVECsGsα和Giα蛋白水平的变化可能是LPS诱导RPMVECs单层通透性增加的机制之一。②甲基强的松龙参与抑制LPS诱导RPMVECs单层通透性增加的作用。
Objective:To observe the influence of lipopolysaccharide(LPS) and methylprednisolone sodium succinate on G protein subunits in rat pulmonary microvascular cell(RPMVECs) membrane.Methods:The level of G protein subunits in RPMVECs membrane was examined by flow cytometry(FCM). Results: ①30 min and 90 min after treatment with 10 μg/ml LPS, the levels of Gsα protein subunits decreased significantly compared with the control group. After methylprednisolone sodium succinate had been given, the effects of LPS on the level of Gsα protein subunits in RPMVECs membrane were inhibited. ②30 min and 90 min after treatment with 10 μg/ml LPS, the level of Giα protein subunits markedly increased compared with the control groups. After methylprednisolone sodium succinate had been given, the effects of LPS on the level of Giα protein subunits in RPMVECs membrane were inhibited. Conclusion: ①Alteration of the levels of Gsα and Giα protein subunits induced by LPS may be an important mechanism in the increase of permeability of RPMVECs induced by LPS. ②Methylprednisolone sodium succinate was involved in inhibiting the increase of permeability of RPMVECs monolayer induced by LPS.
出处
《海军医学杂志》
2001年第4期304-306,共3页
Journal of Navy Medicine
基金
国家自然科学基金项目 ( 396 0 0 0 6 2 )
